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Protonex™ Red 600
Protonex™ Red dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Red dye. The fluorescence of Protonex™ Red dye dramatically increases as pH decreases from neutral to the acidic. The weak fluorescence outside the cell may potentially eliminates the wash steps. Protonex™ Red dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. Protonex™ Red dye is weakly fluorescent outside the cells, but its fluorescence is significantly enhanced in acidic compartments (such as phagosomes, lysosomes and endosomes). This Protonex™ Red enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. It can be also used for multiplexing cellular functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. Protonex™ Red has the spectral properties similar to those of Texas Red, making the common filter set of Texas Red readily available to the assays of Protonex™ Red.
<b>Image of Live HeLa Cells Stained with Protonex™ Red 600.</b> Live HeLa cells were seeded in a 96-well plate and cultured overnight. Following the removal of the growth medium, the cells were washed with Hanks' Balanced Salt Solution (HBSS) and subsequently stained with 0.6 µM Protonex™ Red 600 for 30 minutes at 37°C. After staining, the cells were washed again with HBSS and imaged using fluorescence microscopy with a TRITC filter.
<b>Image of Live HeLa Cells Stained with Protonex™ Red 600.</b> Live HeLa cells were seeded in a 96-well plate and cultured overnight. Following the removal of the growth medium, the cells were washed with Hanks' Balanced Salt Solution (HBSS) and subsequently stained with 0.6 µM Protonex™ Red 600 for 30 minutes at 37°C. After staining, the cells were washed again with HBSS and imaged using fluorescence microscopy with a TRITC filter.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
212071 mg
Price
 
Physical properties
Molecular weight698.94
SolventDMSO
Spectral properties
Excitation (nm)576
Emission (nm)597
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 12, 2025