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Protonex™ Red 600

Protonex™ Red dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Red dye. The fluorescence of Protonex™ Red dye dramatically increases as pH decreases from neutral to the acidic. The weak fluorescence outside the cell may potentially eliminates the wash steps. Protonex™ Red dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. Protonex™ Red dye is weakly fluorescent outside the cells, but its fluorescence is significantly enhanced in acidic compartments (such as phagosomes, lysosomes and endosomes). This Protonex™ Red enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. It can be also used for multiplexing cellular functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. Protonex™ Red has the spectral properties similar to those of Texas Red, making the common filter set of Texas Red readily available to the assays of Protonex™ Red.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 600 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM143.074 µL715.369 µL1.431 mL7.154 mL14.307 mL
5 mM28.615 µL143.074 µL286.148 µL1.431 mL2.861 mL
10 mM14.307 µL71.537 µL143.074 µL715.369 µL1.431 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Citations

View all 6 citations: Citation Explorer
Brain injury accelerates the onset of a reversible age-related microglial phenotype associated with inflammatory neurodegeneration
Authors: Ritzel, Rodney M and Li, Yun and Jiao, Yun and Lei, Zhuofan and Doran, Sarah J and He, Junyun and Shahror, Rami A and Henry, Rebecca J and Khan, Romeesa and Tan, Chunfeng and others,
Journal: Science Advances (2023): eadd1101
Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening
Authors: Zhu, Xinglong and Li, Yi and Yang, Ying and He, Yuting and Gao, Mengyu and Peng, Wanliu and Wu, Qiong and Zhang, Guangyue and Zhou, Yanyan and Chen, Fei and others,
Journal: Materials Today Bio (2022): 100274
Three-Dimensional Microtumor Formation of Infantile Hemangioma-Derived Endothelial Cells for Mechanistic Exploration and Drug Screening
Authors: Li, Yanan and Zhu, Xinglong and Kong, Meng and Chen, Siyuan and Bao, Ji and Ji, Yi
Journal: Pharmaceuticals (2022): 1393
Proton extrusion during oxidative burst in microglia exacerbates pathological acidosis following traumatic brain injury
Authors: Ritzel, Rodney M and He, Junyun and Li, Yun and Cao, Tuoxin and Khan, Niaz and Shim, Bosung and Sabirzhanov, Boris and Aubrecht, Taryn and Stoica, Bogdan A and Faden, Alan I and others,
Journal: Glia (2021): 746--764

References

View all 56 references: Citation Explorer
Monitoring phospholipid dynamics during phagocytosis: application of genetically-encoded fluorescent probes
Authors: Sarantis H, Grinstein S.
Journal: Methods Cell Biol (2012): 429
Phagocytosis and digestion of pH-sensitive fluorescent dye (Eos-FP) transfected E. coli in whole blood assays from patients with severe sepsis and septic shock
Authors: Schreiner L, Huber-Lang M, Weiss ME, Hohmann H, Schmolz M, Schneider EM.
Journal: J Cell Commun Signal (2011): 135
The application of fluorescent probes for the analysis of lipid dynamics during phagocytosis
Authors: Flannagan RS, Grinstein S.
Journal: Methods Mol Biol (2010): 121
Quantification of microsized fluorescent particles phagocytosis to a better knowledge of toxicity mechanisms
Authors: Leclerc L, Boudard D, Pourchez J, Forest V, Sabido O, Bin V, Palle S, Grosseau P, Bernache D, Cottier M.
Journal: Inhal Toxicol (2010): 1091
Analysis of macrophage phagocytosis: quantitative assays of phagosome formation and maturation using high-throughput fluorescence microscopy
Authors: Steinberg BE, Grinstein S.
Journal: Methods Mol Biol (2009): 45
Page updated on December 13, 2024

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Catalog Number21207
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Physical properties

Molecular weight

698.94

Solvent

DMSO

Spectral properties

Excitation (nm)

576

Emission (nm)

597

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
<b>Image of Live HeLa Cells Stained with Protonex™ Red 600.</b> Live HeLa cells were seeded in a 96-well plate and cultured overnight. Following the removal of the growth medium, the cells were washed with Hanks' Balanced Salt Solution (HBSS) and subsequently stained with 0.6 µM Protonex™ Red 600 for 30 minutes at 37°C. After staining, the cells were washed again with HBSS and imaged using fluorescence microscopy with a TRITC filter.
<b>Image of Live HeLa Cells Stained with Protonex™ Red 600.</b> Live HeLa cells were seeded in a 96-well plate and cultured overnight. Following the removal of the growth medium, the cells were washed with Hanks' Balanced Salt Solution (HBSS) and subsequently stained with 0.6 µM Protonex™ Red 600 for 30 minutes at 37°C. After staining, the cells were washed again with HBSS and imaged using fluorescence microscopy with a TRITC filter.
<b>Image of Live HeLa Cells Stained with Protonex™ Red 600.</b> Live HeLa cells were seeded in a 96-well plate and cultured overnight. Following the removal of the growth medium, the cells were washed with Hanks' Balanced Salt Solution (HBSS) and subsequently stained with 0.6 µM Protonex™ Red 600 for 30 minutes at 37°C. After staining, the cells were washed again with HBSS and imaged using fluorescence microscopy with a TRITC filter.
The pH dependent Emission spectra of&nbsp;Protonex&trade; Red 600.