Protonex™ Red 670-Latex Bead Conjugate
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Excitation (nm) | 643 |
Emission (nm) | 660 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Excitation (nm) 643 | Emission (nm) 660 |
Platform
Fluorescence microscope
Excitation | Cy5 filter set |
Emission | Cy5 filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Solvent: | Water |
Solids Content: | 1% in PBS |
Number of Microspheres per mL: | ~4e+10 |
Ex/Em: | 643/660 nm |
Mean Diameter: | 0.72 µm |
SAMPLE EXPERIMENTAL PROTOCOL
The following is a recommended protocol for granulocytes. This protocol only provides a guideline and should be modified
according to your specific experimental conditions.
Prepare cells as desired. For example, prepare the granulocytes at 107 cells/mL with Hanks and 20 mM Hepes buffer (HHBS), and add 100 μL to a polypropylene tube.
Note: Each cell line should be evaluated on an individual basis to determine the optimal cell density.
Add 1-10 μL of the Protonex™ Red 670-Latex Bead Conjugate to the tube and incubate with gentle shaking for 30 minutes at 37˚C.
Note: Each cell line should be evaluated on an individual basis to determine the optimal incubation time.
Prepare an identical sample that is incubated at 4˚C and label it as a control.
At the end of the 30-minute incubation, stop phagocytosis by adding 2mL of ice-cold HHBS and mix well.
Wash the cells 2 times with cold HBSS.
Resuspend the cells in 500 μL of cold HBSS, keep the samples at 4˚C, and analyze immediately using a fluorescence microscope equipped with a Cy5 filter set.
Note: For fluorescence microplate readers, monitor the fluorescence intensity at Ex/Em = 640/680 nm (Cutoff = 660 nm).
References
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Journal: Advanced materials (Deerfield Beach, Fla.) (2023): e2302292
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Journal: Biomaterials science (2023): 7114-7123
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Journal: Journal of clinical medicine (2023)
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Journal: Bio-protocol (2023): e4800
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Journal: Nanoscale (2022): 8000-8011