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Psoralen MOP Maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 410.38 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| Psoralen MOP Succinimidyl Ester |
| Psoralen MOP Azide |
| Psoralen MOP Alkyne |
| Overview |  SDSProtocol |
Molecular weight 410.38 |
Psoralens and their derivatives (such as 8-MOP and 4,5’8-TMP) are well known to have unique crosslinking features to DNA. However, psoralen monomers do not have sequence-specific crosslinking ability with a target DNA. Nakao et al found that 5-MOP more effectively crosslinks DNA compared to the well-known 8-MOP (https://doi.org/10.1002/cbic.202200789). Psoralen MOP Maleimide is a thiol-reactive 5-MOP derivative. It is an excellent building block for preparing MOP-labeled oligos from the readily available thiol-modified oligos. The 5-MOP-conjugated oligonucleotides can be used for sequence-specific crosslinking with a target DNA, thus enabling the application of psoralen-conjugated molecules in gene transcription inhibition, gene knockout, and other genomic applications. Psoralen MOP Maleimide may also be used for preparing site-specific DNA/RNA probes via the conjugations with thiol-containing biomolecules such as antibodies.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Psoralen MOP Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 243.677 µL | 1.218 mL | 2.437 mL | 12.184 mL | 24.368 mL |
| 5 mM | 48.735 µL | 243.677 µL | 487.353 µL | 2.437 mL | 4.874 mL |
| 10 mM | 24.368 µL | 121.838 µL | 243.677 µL | 1.218 mL | 2.437 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Psoralen MOP Maleimide is a thiol-reactive 5-MOP derivative. It is an excellent building block for preparing MOP-labeled oligos from the readily available thiol-modified oligos.
References
View all 50 references: Citation Explorer
The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling.
Authors: Kouzine, Fedor and Baranello, Laura and Levens, David
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 95-108
Authors: Kouzine, Fedor and Baranello, Laura and Levens, David
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 95-108
Differential roles for Chk1 and FANCD2 in ATR-mediated signalling for psoralen photoactivation-induced senescence.
Authors: Hovest, Miriam G and Krieg, Thomas and Herrmann, Gernot
Journal: Experimental dermatology (2011): 883-9
Authors: Hovest, Miriam G and Krieg, Thomas and Herrmann, Gernot
Journal: Experimental dermatology (2011): 883-9
Tanning caused by psoralen-photochemotherpy in Indian skin.
Authors: Gupta, Somesh and Ajith, C
Journal: Indian journal of dermatology, venereology and leprology (2009): 76-7
Authors: Gupta, Somesh and Ajith, C
Journal: Indian journal of dermatology, venereology and leprology (2009): 76-7
Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase and persistent DNA damage foci at telomeres.
Authors: Hovest, Miriam Grosse and Brüggenolte, Nicole and Hosseini, Kijawasch Shah and Krieg, Thomas and Herrmann, Gernot
Journal: Molecular biology of the cell (2006): 1758-67
Authors: Hovest, Miriam Grosse and Brüggenolte, Nicole and Hosseini, Kijawasch Shah and Krieg, Thomas and Herrmann, Gernot
Journal: Molecular biology of the cell (2006): 1758-67
The contribution of medical physics to the development of psoralen photochemotherapy (PUVA) in the UK: a personal reminiscence.
Authors: Diffey, Brian
Journal: Physics in medicine and biology (2006): R229-44
Authors: Diffey, Brian
Journal: Physics in medicine and biology (2006): R229-44
Diffuse cutaneous mastocytosis treated with psoralen photochemotherapy: case report and review of the literature.
Authors: Kinsler, V A and Hawk, J L M and Atherton, D J
Journal: The British journal of dermatology (2005): 179-80
Authors: Kinsler, V A and Hawk, J L M and Atherton, D J
Journal: The British journal of dermatology (2005): 179-80
Mechanism of site-specific psoralen photoadducts formation in triplex DNA directed by psoralen-conjugated oligonucleotides.
Authors: Ping, Yueh-Hsin and Rana, Tariq M
Journal: Biochemistry (2005): 2501-9
Authors: Ping, Yueh-Hsin and Rana, Tariq M
Journal: Biochemistry (2005): 2501-9
Audit of the use of psoralen photochemotherapy (PUVA) and narrowband UVB phototherapy in the treatment of psoriasis.
Authors: Yones, S S and Palmer, R A and Kuno, Y and Hawk, J L M
Journal: The Journal of dermatological treatment (2005): 108-12
Authors: Yones, S S and Palmer, R A and Kuno, Y and Hawk, J L M
Journal: The Journal of dermatological treatment (2005): 108-12
Psoralen photocrosslinking, a tool to study the chromatin structure of RNA polymerase I--transcribed ribosomal genes.
Authors: Toussaint, Martin and Levasseur, Geneviève and Tremblay, Maxime and Paquette, Michel and Conconi, Antonio
Journal: Biochemistry and cell biology = Biochimie et biologie cellulaire (2005): 449-59
Authors: Toussaint, Martin and Levasseur, Geneviève and Tremblay, Maxime and Paquette, Michel and Conconi, Antonio
Journal: Biochemistry and cell biology = Biochimie et biologie cellulaire (2005): 449-59
Treatment of persistent severe atopic dermatitis in 113 Japanese patients with oral psoralen photo-chemotherapy.
Authors: Uetsu, Naoko and Horio, Takeshi
Journal: The Journal of dermatology (2003): 450-7
Authors: Uetsu, Naoko and Horio, Takeshi
Journal: The Journal of dermatology (2003): 450-7