Purified Azide Free Mouse Anti-human TNF α Antibody *MAb1, monoclonal*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Overview | Protocol |
See also: Antibodies and Proteomics
Other names | Clone MAb1 | Host mouse | Reactivity human | Application WB; ELISA; FUNC |
Tumor necrosis factor (also known as TNF-α, TNF-a, Tumor necrosis factor ligand superfamily member 2 or Cachectin) is a transmembrane protein with a molecular weight of 26 kDa, found in the phagocytic cup, integral component of plasma membrane and recycling endosome of cells. In humans, TNF-α has been thought to be involved with important functions like cytokine activity. It is the subject of extensive research because of the fact that it acts in the lipopolysaccharide-mediated signaling pathway, intrinsic apoptotic signaling pathway in response to DNA damage and extrinsic apoptotic signaling pathway via death domain receptors. Sequencing of TNF-α has exemplified it contains 2 types of conserved structural units: extracellular and cytoplasmic domain. TNF-α is a suppressor of fat cell differentiation, myosin-light-chain-phosphatase activity and interleukin-6 production while also is a positive regulator of interleukin-8 production, ERK1 and ERK2 cascade and protein catabolic process. It is an integral part of organismal processes, in particular, microglial cell activation, JNK cascade and cortical actin cytoskeleton organization. TNF-α binds to protease, transcription regulatory region sequence-specific DNA and identical protein. It has been found to be involved in establishment of endothelial barrier, endothelial cell apoptotic process and transcription by RNA polymerase II. TNF-α is clinically significant because abnormalities in its function have been closely linked to diseases such as psoriatic arthritis.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of TNF α antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?