Purified Mouse Anti-human TNF α Antibody *MAb1, monoclonal*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Overview | Protocol |
See also: Antibodies and Proteomics
Other names | Clone MAb1 | Host mouse | Reactivity human | Application WB; ELISA |
Tumor necrosis factor (also known as TNF-α, TNF-a, Tumor necrosis factor ligand superfamily member 2 or Cachectin) is a transmembrane protein with a molecular weight of 26 kDa, found in the phagocytic cup, integral component of plasma membrane and recycling endosome of cells. In humans, TNF-α has been thought to be involved with important functions like cytokine activity. It is the subject of extensive research because of the fact that it acts in the lipopolysaccharide-mediated signaling pathway, intrinsic apoptotic signaling pathway in response to DNA damage and extrinsic apoptotic signaling pathway via death domain receptors. Sequencing of TNF-α has exemplified it contains 2 types of conserved structural units: extracellular and cytoplasmic domain. TNF-α is a suppressor of fat cell differentiation, myosin-light-chain-phosphatase activity and interleukin-6 production while also is a positive regulator of interleukin-8 production, ERK1 and ERK2 cascade and protein catabolic process. It is an integral part of organismal processes, in particular, microglial cell activation, JNK cascade and cortical actin cytoskeleton organization. TNF-α binds to protease, transcription regulatory region sequence-specific DNA and identical protein. It has been found to be involved in establishment of endothelial barrier, endothelial cell apoptotic process and transcription by RNA polymerase II. TNF-α is clinically significant because abnormalities in its function have been closely linked to diseases such as psoriatic arthritis.
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of TNF α antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?