Name: Rabbit Anti-Alexa Fluor 350 Antibody
Brand: AAT Bioquest
SKU: 46620
Price: 148 USD
Availability: InStock

Rabbit Anti-Alexa Fluor 350 Antibody

Rabbit Anti-Alexa Fluor 350 Antibody is a polyclonal antibody for detecting Alexa Fluor® 350 conjugates.

Polyclonal Format: Rabbit-derived with broad epitope recognition.
High Affinity: Strong binding to Alexa Fluor® 350-labeled targets.
Cross-Reactive: May detect iFluor® 350, AMCA, and Cascade Blue®.
Assay Ready: Works in ELISA, microscopy, and blue-channel fluorescence applications.

Indirect ELISA of Rabbit Anti-Alexa Fluor 350 Antibody. Microplates were coated with 100 ng/well of BSA-Alexa Fluor 350 or BSA (control). Coating, blocking, and washing steps followed standard ELISA procedures. Rabbit Anti-Alexa Fluor 350 Antibody (Cat# 46620) was serially diluted threefold from 100 ng/well and incubated for 40 minutes. After washing, HRP-labeled goat anti-rabbit IgG (Cat# 16793) (100 ng/mL in PBST; 100 µL/well) was added and incubated at room temperature for 40 minutes. Bound HRP activity was detected using ReadiUse™ TMB Substrate Solution (Cat# 11012), and absorbance was measured at 450 nm.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
46620	1 mg	Price

Overview

Rabbit Anti-Alexa Fluor 350 Antibody is a polyclonal antibody that specifically binds to Alexa Fluor® 350-conjugated molecules. It is designed for detecting Alexa Fluor® 350-labeled antibodies, proteins, or other probes in fluorescence-based assays. The antibody may cross-react with related blue fluorophores such as iFluor® 350, AMCA, and Cascade Blue®. It is ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection. Alexa Fluor® and Cascade Blue® are registered trademarks of Thermo Fisher Scientific.

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of Alexa Fluor 350 coupled to carrier protein antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

References

View all 5 references: Citation Explorer

Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells.

Authors:

Dolton, Garry and Zervoudi, Efthalia and Rius, Cristina and Wall, Aaron and Thomas, Hannah L and Fuller, Anna and Yeo, Lorraine and Legut, Mateusz and Wheeler, Sophie and Attaf, Meriem and Chudakov, Dmitriy M and Choy, Ernest and Peakman, Mark and Sewell, Andrew K

Journal:

Frontiers in immunology (2018): 1378

The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and Their In Vitro Equivalents.

Authors:

Vremec, David

Journal:

Methods in molecular biology (Clifton, N.J.) (2016): 61-87

Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs.

Authors:

Tungatt, Katie and Bianchi, Valentina and Crowther, Michael D and Powell, Wendy E and Schauenburg, Andrea J and Trimby, Andrew and Donia, Marco and Miles, John J and Holland, Christopher J and Cole, David K and Godkin, Andrew J and Peakman, Mark and Straten, Per Thor and Svane, Inge Marie and Sewell, Andrew K and Dolton, Garry

Journal:

Journal of immunology (Baltimore, Md. : 1950) (2015): 463-74

The purification of large numbers of antigen presenting dendritic cells from mouse spleen.

Authors:

Vremec, David and Segura, Elodie

Journal:

Methods in molecular biology (Clifton, N.J.) (2013): 327-350

Immunomagnetic purification of T cell subpopulations.

Authors:

Horgan, Kevin and Shaw, Stephen and Boirivant, Monica

Journal:

Current protocols in immunology (2009): 7.4.1-7.4.9

Physical properties

Solvent

Water

Antibody properties

Host	Rabbit
Application	IF, ELISA, Flow Cytometry (FACS)

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Fax
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Page updated on July 14, 2026