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Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 647-Labeled
Product key features
- Direct Detection: iFluor® 647 enables one-step visualization of Alexa Fluor® 350 targets.
- Spectrally Distinct: Far-red emission avoids overlap with blue-channel fluorophores.
- Polyclonal Format: Rabbit-derived with broad epitope recognition.
- Multipurpose Use: Ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection.
Product description
Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 647-Labeled is a polyclonal antibody that specifically binds to Alexa Fluor® 350-conjugated molecules and is directly labeled with iFluor® 647, a far-red fluorophore with excitation/emission maxima of ~656/670 nm. This configuration allows for one-step fluorescence detection without the need for secondary antibodies.
The antibody may cross-react with structurally similar fluorophores such as iFluor® 350, AMCA, and Cascade Blue®. The far-red emission of iFluor® 647 avoids spectral overlap with blue fluorophores, making this reagent especially effective in signal amplification, conjugation verification, and multiplexed fluorescence detection making it a versatile tool for imaging and analytical workflows.
Alexa Fluor® and Cascade Blue® are registered trademarks of Thermo Fisher Scientific.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 350-Labeled | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 488-Labeled | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 555-Labeled | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 750-Labeled | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
| Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 350-Labeled | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 647-Labeled | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
| Rabbit Anti-Alexa Fluor 555 Antibody, iFluor® 350-Labeled | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| Rabbit Anti-Alexa Fluor 555 Antibody, iFluor® 647-Labeled | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
| Rabbit Anti-Alexa Fluor 594 Antibody, iFluor® 350-Labeled | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
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References
Authors: Dolton, Garry and Zervoudi, Efthalia and Rius, Cristina and Wall, Aaron and Thomas, Hannah L and Fuller, Anna and Yeo, Lorraine and Legut, Mateusz and Wheeler, Sophie and Attaf, Meriem and Chudakov, Dmitriy M and Choy, Ernest and Peakman, Mark and Sewell, Andrew K
Journal: Frontiers in immunology (2018): 1378
Authors: Vremec, David
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 61-87
Authors: Tungatt, Katie and Bianchi, Valentina and Crowther, Michael D and Powell, Wendy E and Schauenburg, Andrea J and Trimby, Andrew and Donia, Marco and Miles, John J and Holland, Christopher J and Cole, David K and Godkin, Andrew J and Peakman, Mark and Straten, Per Thor and Svane, Inge Marie and Sewell, Andrew K and Dolton, Garry
Journal: Journal of immunology (Baltimore, Md. : 1950) (2015): 463-74
Authors: Vremec, David and Segura, Elodie
Journal: Methods in molecular biology (Clifton, N.J.) (2013): 327-350
Authors: Horgan, Kevin and Shaw, Stephen and Boirivant, Monica
Journal: Current protocols in immunology (2009): 7.4.1-7.4.9
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