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Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 350-Labeled
Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 350-Labeled is a polyclonal antibody labeled with iFluor® 350 for direct detection of Alexa Fluor® 488 conjugates.
- Direct Detection: iFluor® 350 label enables one-step fluorescence detection of Alexa Fluor® 488 targets.
- Spectrally Distinct: Blue fluorescence avoids overlap with green-channel dyes like Alexa Fluor® 488.
- Polyclonal Design: Rabbit-derived for broad recognition of Alexa Fluor® 488-conjugated molecules.
- Multipurpose Use: Ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection.
Product description
Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 350-Labeled is a polyclonal antibody that specifically binds to Alexa Fluor® 488-conjugated targets and is directly labeled with iFluor® 350, a bright, UV-excitable blue fluorophore. This format allows for direct detection of Alexa Fluor 488-labeled biomolecules without the need for a secondary antibody.
The antibody may cross-react with structurally related dyes such as iFluor® 488, ATTO 488, and rhodamine 110. Its spectral separation from Alexa Fluor 488 makes it ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. iFluor® is a registered trademark of AAT Bioquest, Inc.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 488-Labeled | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 555-Labeled | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 647-Labeled | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
| Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 750-Labeled | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
| Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 350-Labeled | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 555-Labeled | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 647-Labeled | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
| Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 750-Labeled | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
| Rabbit Anti-Alexa Fluor 555 Antibody, iFluor® 350-Labeled | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
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References
View all 5 references: Citation Explorer
Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells.
Authors: Dolton, Garry and Zervoudi, Efthalia and Rius, Cristina and Wall, Aaron and Thomas, Hannah L and Fuller, Anna and Yeo, Lorraine and Legut, Mateusz and Wheeler, Sophie and Attaf, Meriem and Chudakov, Dmitriy M and Choy, Ernest and Peakman, Mark and Sewell, Andrew K
Journal: Frontiers in immunology (2018): 1378
Authors: Dolton, Garry and Zervoudi, Efthalia and Rius, Cristina and Wall, Aaron and Thomas, Hannah L and Fuller, Anna and Yeo, Lorraine and Legut, Mateusz and Wheeler, Sophie and Attaf, Meriem and Chudakov, Dmitriy M and Choy, Ernest and Peakman, Mark and Sewell, Andrew K
Journal: Frontiers in immunology (2018): 1378
The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and Their In Vitro Equivalents.
Authors: Vremec, David
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 61-87
Authors: Vremec, David
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 61-87
Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs.
Authors: Tungatt, Katie and Bianchi, Valentina and Crowther, Michael D and Powell, Wendy E and Schauenburg, Andrea J and Trimby, Andrew and Donia, Marco and Miles, John J and Holland, Christopher J and Cole, David K and Godkin, Andrew J and Peakman, Mark and Straten, Per Thor and Svane, Inge Marie and Sewell, Andrew K and Dolton, Garry
Journal: Journal of immunology (Baltimore, Md. : 1950) (2015): 463-74
Authors: Tungatt, Katie and Bianchi, Valentina and Crowther, Michael D and Powell, Wendy E and Schauenburg, Andrea J and Trimby, Andrew and Donia, Marco and Miles, John J and Holland, Christopher J and Cole, David K and Godkin, Andrew J and Peakman, Mark and Straten, Per Thor and Svane, Inge Marie and Sewell, Andrew K and Dolton, Garry
Journal: Journal of immunology (Baltimore, Md. : 1950) (2015): 463-74
The purification of large numbers of antigen presenting dendritic cells from mouse spleen.
Authors: Vremec, David and Segura, Elodie
Journal: Methods in molecular biology (Clifton, N.J.) (2013): 327-350
Authors: Vremec, David and Segura, Elodie
Journal: Methods in molecular biology (Clifton, N.J.) (2013): 327-350
Immunomagnetic purification of T cell subpopulations.
Authors: Horgan, Kevin and Shaw, Stephen and Boirivant, Monica
Journal: Current protocols in immunology (2009): 7.4.1-7.4.9
Authors: Horgan, Kevin and Shaw, Stephen and Boirivant, Monica
Journal: Current protocols in immunology (2009): 7.4.1-7.4.9
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