Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 488-Labeled

Product key features

Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 488-Labeled is a polyclonal antibody labeled with iFluor® 488 for highly sensitive detection of Alexa Fluor® 488 conjugates.

Direct Detection: Enables one-step analysis without secondary antibodies.
Spectrally Matched: Fluorophore pairing enhances signal strength and simplifies detection within the orange emission channel.
Polyclonal Format: Rabbit-derived for broad recognition of Alexa Fluor® 555-conjugated molecules.
Multipurpose Use: Ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection

Product description

Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 488-Labeled is a polyclonal antibody that specifically binds to Alexa Fluor® 488-conjugated targets and is directly labeled with iFluor® 488, a green-emitting fluorophore with excitation around 490 nm. This direct labeling format allows sensitive, single-step fluorescence detection, minimizing background and improving workflow efficiency.

The antibody may cross-react with structurally similar dyes such as iFluor® 488, ATTO 488, and rhodamine 110. It is ideal for signal amplification in low-abundance target detection, verifying dye conjugation efficiency, and performing multiplex fluorescence assays in various applications.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 350-Labeled	345	450	20000¹	0.95¹	0.83	0.23
Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 555-Labeled	491	516	75000¹	0.9¹	0.21	0.11
Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 647-Labeled	656	670	250000¹	0.25¹	0.03	0.03
Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 750-Labeled	757	779	275000¹	0.12¹	0.044	0.039
Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 488-Labeled	491	516	75000¹	0.9¹	0.21	0.11
Rabbit Anti-Alexa Fluor 555 Antibody, iFluor® 488-Labeled	491	516	75000¹	0.9¹	0.21	0.11
Rabbit Anti-Alexa Fluor 594 Antibody, iFluor® 488-Labeled	491	516	75000¹	0.9¹	0.21	0.11
Rabbit Anti-Alexa Fluor 647 Antibody, iFluor® 488-Labeled	491	516	75000¹	0.9¹	0.21	0.11
Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 488-Labeled	491	516	75000¹	0.9¹	0.21	0.11

References

View all 5 references: Citation Explorer

Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells.
Authors: Dolton, Garry and Zervoudi, Efthalia and Rius, Cristina and Wall, Aaron and Thomas, Hannah L and Fuller, Anna and Yeo, Lorraine and Legut, Mateusz and Wheeler, Sophie and Attaf, Meriem and Chudakov, Dmitriy M and Choy, Ernest and Peakman, Mark and Sewell, Andrew K
Journal: Frontiers in immunology (2018): 1378

The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and Their In Vitro Equivalents.
Authors: Vremec, David
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 61-87

Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs.
Authors: Tungatt, Katie and Bianchi, Valentina and Crowther, Michael D and Powell, Wendy E and Schauenburg, Andrea J and Trimby, Andrew and Donia, Marco and Miles, John J and Holland, Christopher J and Cole, David K and Godkin, Andrew J and Peakman, Mark and Straten, Per Thor and Svane, Inge Marie and Sewell, Andrew K and Dolton, Garry
Journal: Journal of immunology (Baltimore, Md. : 1950) (2015): 463-74

The purification of large numbers of antigen presenting dendritic cells from mouse spleen.
Authors: Vremec, David and Segura, Elodie
Journal: Methods in molecular biology (Clifton, N.J.) (2013): 327-350

Immunomagnetic purification of T cell subpopulations.
Authors: Horgan, Kevin and Shaw, Stephen and Boirivant, Monica
Journal: Current protocols in immunology (2009): 7.4.1-7.4.9

Page updated on August 1, 2025

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Catalog Number	46513
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Physical properties

Solvent

PBS

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹