Rabbit Anti-Alexa Fluor 647 Antibody, iFluor® 750-Labeled

Product key features

Rabbit Anti-Alexa Fluor 647 Antibody, iFluor® 750-Labeled is a polyclonal antibody labeled with iFluor® 750 for direct detection of Alexa Fluor® 647 conjugates.

Direct Detection: iFluor® 750 enables one-step fluorescence detection of Alexa Fluor® 647 targets.
Spectrally Distinct: Near-infrared emission avoids overlap with visible fluorophores.
Polyclonal Format: Rabbit-derived with broad recognition of Alexa Fluor® 647-conjugated molecules.
Multipurpose Use: Ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection.

Product description

Rabbit Anti-Alexa Fluor 647 Antibody, iFluor® 750-Labeled is a polyclonal antibody that specifically binds to Alexa Fluor® 647-conjugated molecules and is directly labeled with iFluor® 750, a near-infrared fluorophore with excitation/emission of ~752/778 nm. This direct labeling format allows single-step detection of Alexa Fluor® 647-labeled targets without requiring secondary antibodies.

The antibody may cross-react with structurally similar fluorophores such as iFluor® 647, Cy5, ATTO 647, and DyLight® 650. Its near-infrared emission reduces background and is ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection

Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

Spectrum

Open in Advanced Spectrum Viewer

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Rabbit Anti-Alexa Fluor 647 Antibody, iFluor® 350-Labeled	345	450	20000¹	0.95¹	0.83	0.23
Rabbit Anti-Alexa Fluor 647 Antibody, iFluor® 488-Labeled	491	516	75000¹	0.9¹	0.21	0.11
Rabbit Anti-Alexa Fluor 647 Antibody, iFluor® 555-Labeled	557	570	100000¹	0.64¹	0.23	0.14
Rabbit Anti-Alexa Fluor 647 Antibody, iFluor® 647-Labeled	656	670	250000¹	0.25¹	0.03	0.03
Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 647-Labeled	656	670	250000¹	0.25¹	0.03	0.03
Rabbit Anti-Alexa Fluor 350 Antibody, iFluor® 750-Labeled	757	779	275000¹	0.12¹	0.044	0.039
Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 647-Labeled	656	670	250000¹	0.25¹	0.03	0.03
Rabbit Anti-Alexa Fluor 488 Antibody, iFluor® 750-Labeled	757	779	275000¹	0.12¹	0.044	0.039
Rabbit Anti-Alexa Fluor 555 Antibody, iFluor® 647-Labeled	656	670	250000¹	0.25¹	0.03	0.03
Rabbit Anti-Alexa Fluor 555 Antibody, iFluor® 750-Labeled	757	779	275000¹	0.12¹	0.044	0.039
Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 350-Labeled	345	450	20000¹	0.95¹	0.83	0.23
Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 488-Labeled	491	516	75000¹	0.9¹	0.21	0.11
Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 555-Labeled	557	570	100000¹	0.64¹	0.23	0.14
Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 750-Labeled	757	779	275000¹	0.12¹	0.044	0.039
Show More (5)

References

View all 5 references: Citation Explorer

Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells.
Authors: Dolton, Garry and Zervoudi, Efthalia and Rius, Cristina and Wall, Aaron and Thomas, Hannah L and Fuller, Anna and Yeo, Lorraine and Legut, Mateusz and Wheeler, Sophie and Attaf, Meriem and Chudakov, Dmitriy M and Choy, Ernest and Peakman, Mark and Sewell, Andrew K
Journal: Frontiers in immunology (2018): 1378

The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and Their In Vitro Equivalents.
Authors: Vremec, David
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 61-87

Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs.
Authors: Tungatt, Katie and Bianchi, Valentina and Crowther, Michael D and Powell, Wendy E and Schauenburg, Andrea J and Trimby, Andrew and Donia, Marco and Miles, John J and Holland, Christopher J and Cole, David K and Godkin, Andrew J and Peakman, Mark and Straten, Per Thor and Svane, Inge Marie and Sewell, Andrew K and Dolton, Garry
Journal: Journal of immunology (Baltimore, Md. : 1950) (2015): 463-74

The purification of large numbers of antigen presenting dendritic cells from mouse spleen.
Authors: Vremec, David and Segura, Elodie
Journal: Methods in molecular biology (Clifton, N.J.) (2013): 327-350

Immunomagnetic purification of T cell subpopulations.
Authors: Horgan, Kevin and Shaw, Stephen and Boirivant, Monica
Journal: Current protocols in immunology (2009): 7.4.1-7.4.9

Page updated on August 1, 2025

Ordering information

Price
Unit size
Catalog Number	46572
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Request quotation

Physical properties

Solvent

PBS

Spectral properties

Correction Factor (260 nm)	0.044
Correction Factor (280 nm)	0.039
Correction Factor (565 nm)	0.0250
Correction Factor (650 nm)	0.1413
Extinction coefficient (cm ^-1 M ^-1)	275000¹
Excitation (nm)	757
Emission (nm)	779
Quantum yield	0.12¹