Name: Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 350-Labeled
Brand: AAT Bioquest
SKU: 46592
Price: 95 USD
Availability: InStock

Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 350-Labeled

Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 350-Labeled is a polyclonal antibody labeled with iFluor® 350 for detecting Alexa Fluor® 750 conjugates.

Direct Detection: iFluor® 350 enables one-step visualization of Alexa Fluor® 750 targets.
Spectrally Distinct: Blue emission avoids overlap with near-infrared fluorophores.
Polyclonal Format: Rabbit-derived with broad target recognition.
Multiplex Ready: Suitable for multicolor assays, flow cytometry, and low-background imaging.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
46592	100 ug	Price

Overview

Rabbit Anti-Alexa Fluor 750 Antibody, iFluor® 350-Labeled is a polyclonal antibody that specifically binds to Alexa Fluor® 750-conjugated molecules and is directly labeled with iFluor® 350, a UV-excitable blue fluorophore with excitation/emission of ~346/442 nm. This direct labeling format enables one-step detection of Alexa Fluor® 750-labeled targets without the need for secondary antibodies. The antibody may cross-react with structurally related dyes such as iFluor® 750, ATTO 740, and DyLight® 755. Its blue emission is well separated from near-infrared signals, making it ideal for multiplex assays and low-background imaging workflows. Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of Alexa Fluor 750 coupled to carrier protein antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

References

View all 5 references: Citation Explorer

Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells.

Authors:

Dolton, Garry and Zervoudi, Efthalia and Rius, Cristina and Wall, Aaron and Thomas, Hannah L and Fuller, Anna and Yeo, Lorraine and Legut, Mateusz and Wheeler, Sophie and Attaf, Meriem and Chudakov, Dmitriy M and Choy, Ernest and Peakman, Mark and Sewell, Andrew K

Journal:

Frontiers in immunology (2018): 1378

The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and Their In Vitro Equivalents.

Authors:

Vremec, David

Journal:

Methods in molecular biology (Clifton, N.J.) (2016): 61-87

Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs.

Authors:

Tungatt, Katie and Bianchi, Valentina and Crowther, Michael D and Powell, Wendy E and Schauenburg, Andrea J and Trimby, Andrew and Donia, Marco and Miles, John J and Holland, Christopher J and Cole, David K and Godkin, Andrew J and Peakman, Mark and Straten, Per Thor and Svane, Inge Marie and Sewell, Andrew K and Dolton, Garry

Journal:

Journal of immunology (Baltimore, Md. : 1950) (2015): 463-74

The purification of large numbers of antigen presenting dendritic cells from mouse spleen.

Authors:

Vremec, David and Segura, Elodie

Journal:

Methods in molecular biology (Clifton, N.J.) (2013): 327-350

Immunomagnetic purification of T cell subpopulations.

Authors:

Horgan, Kevin and Shaw, Stephen and Boirivant, Monica

Journal:

Current protocols in immunology (2009): 7.4.1-7.4.9

Physical properties

Solvent

Water

Antibody properties

Host	rabbit
Application	IF; ELISA; Flow Cytometry (FACS)

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 17, 2025