Name: Rabbit Anti-Coumarin Antibody
Brand: AAT Bioquest
SKU: 46780
Price: 148 USD
Availability: InStock

Rabbit Anti-Coumarin Antibody

Rabbit Anti-Coumarin Antibody is a polyclonal antibody for detecting coumarin-based fluorophores.

Polyclonal Format: Rabbit-derived with broad epitope recognition of coumarin-labeled molecules.
High Affinity: Strong binding to coumarin and structurally related dyes.
Cross-Reactive: May detect AMC, AMCA, and other coumarin analogs.
Multipurpose Use: Ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection.

Indirect ELISA of Rabbit Anti-Coumarin Antibody. Microplates were coated with 100 ng/well of BSA-Coumarin or BSA (control). Coating, blocking, and washing steps followed standard ELISA procedures. Rabbit Anti-Coumarin Antibody (Cat# 46780) was serially diluted threefold from 100 ng/well and incubated for 40 minutes. After washing, HRP-labeled goat anti-rabbit IgG (Cat# 16793) (100 ng/mL in PBST; 100 µL/well) was added and incubated at room temperature for 40 minutes. Bound HRP activity was detected using ReadiUse™ TMB Substrate Solution (Cat# 11012), and absorbance was measured at 450 nm.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
46780	1 mg	Price

Overview

Rabbit Anti-Coumarin Antibody is a polyclonal antibody that specifically recognizes coumarin and coumarin-derived fluorophores commonly used as blue-emitting labels in biological assays. It provides high-affinity binding for detecting coumarin-conjugated biomolecules. The antibody may cross-react with related blue-emitting dyes such as AMC (7-amino-4-methylcoumarin), AMCA, and other coumarin derivatives. It is ideal for signal amplification in detecting low-abundance coumarin conjugates, validating dye incorporation, and enabling multiplexed fluorescence assays.

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of Coumarin coupled to carrier protein antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

References

View all 50 references: Citation Explorer

Novel bispecific monoclonal antibody-based dual-analyte immunochromatographic strip for the detection of aflatoxin B1 and ochratoxin A in cereal samples.

Authors:

Wu, Shih-Wei and Tai, Wei Hong and Hsieh, Chia-Yu and Liu, Biing-Hui and Yu, Feng-Yih

Journal:

Food chemistry (2025): 144762

Real world application of the 2023 ACR/EULAR antiphospholipid antibody syndrome classification criteria in a pharmacist directed anticoagulation clinic.

Authors:

DeFazio, Catherine and Horvath, Lara and Morgan, Jennifer and Murphy, Meaghan B and Shtoyko, Ashley N and Phillips, Elizabeth M

Journal:

Lupus (2025): 34-38

Secondary prevention with antiplatelet medications in patients with antiphospholipid antibody-related stroke.

Authors:

Yang, Wookjin and Chung, Matthew and Ha, Jiyeon and Kang, Dong-Wan and Lee, Eung-Joon and Jeong, Han-Yeong and Kim, Jeong-Min and Jung, Keun-Hwa and Lee, Seung-Hoon

Journal:

Scientific reports (2025): 7282

Biomimetic immobilization of α-glucosidase inspired by antibody-antigen specific recognition for catalytic preparation of 4-methylumbelliferone.

Authors:

Guo, Shuang and Liu, Shuo and Liu, Chang and Wang, Yi and Gu, Dongyu and Tian, Jing and Yang, Yi

Journal:

International journal of biological macromolecules (2024): 131697

An Extremely Highly Sensitive ELISA in pg mL-1 Level Based on a Newly Produced Monoclonal Antibody for the Detection of Ochratoxin A in Food Samples.

Authors:

Ren, Yexuan and Tian, Ruwen and Wang, Ting and Cao, Junlin and Li, Jianguo and Deng, Anping

Journal:

Molecules (Basel, Switzerland) (2023)

Physical properties

Solvent

Water

Antibody properties

Host	Rabbit
Application	IF, ELISA, Flow Cytometry (FACS)

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Fax
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Page updated on May 9, 2026