Name: Rabbit Anti-Fluorescein [FITC] Antibody
Brand: AAT Bioquest
SKU: 46750
Price: 148 USD
Availability: InStock

Rabbit Anti-Fluorescein Antibody

FITC

Rabbit Anti-Fluorescein [FITC] Antibody is a polyclonal antibody designed to detect FITC-labeled targets.

High Sensitivity: Binds FITC with high affinity for detecting low-abundance targets.
Polyclonal Format: Rabbit-derived with broad reactivity to fluorescein and analogs.
Cross-Reactive: May recognize Alexa Fluor® 488 and DyLight® 488.
Multipurpose Use: Ideal for signal amplification, conjugation verification, and multiplexed fluorescence detection.

Indirect ELISA of Rabbit Anti-Fluorescein [FITC] Antibody. Microplates were coated with 100 ng/well of BSA-FITC or BSA (control). Coating, blocking, and washing steps followed standard ELISA procedures. Rabbit Anti-Fluorescein [FITC] Antibody
(Cat# 46750) was serially diluted threefold from 100 ng/well and incubated for 40 minutes. After washing, HRP-labeled goat anti-rabbit IgG (Cat# 16793) (100 ng/mL in PBST; 100 µL/well) was added and incubated at room temperature for 40 minutes. Bound HRP activity was detected using ReadiUse™ TMB Substrate Solution (Cat# 11012), and absorbance was measured at 450 nm.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
46750	1 mg	Price

Overview

Rabbit Anti-Fluorescein [FITC] Antibody is a polyclonal antibody that specifically binds to fluorescein-based dyes such as FITC (fluorescein isothiocyanate), a widely used green fluorophore in molecular biology. This antibody enables detection of FITC-labeled biomolecules including antibodies, oligonucleotides, and proteins. It may cross-react with structurally related dyes like Alexa Fluor® 488, and DyLight® 488. The antibody is ideal for signal amplification in applications where FITC-labeled targets are present at low levels, and is suitable for use in various techniques. Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of FITC coupled to carrier protein antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

References

View all 50 references: Citation Explorer

Artificial Internalizing Receptors: Intracellular Delivery of Cargo Through Bio-Orthogonal Recognition.

Authors:

Søgaard, Ane Bretschneider and Hansson, Rikke Fabech and Tvilum, Anne Selch and Zelikin, Alexander N

Journal:

Advanced healthcare materials (2024): e2402472

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii.

Authors:

Kanapathy, Swarnaletchumi and Obande, Godwin Attah and Chuah, Candy and Shueb, Rafidah Hanim and Yean, Chan Yean and Banga Singh, Kirnpal Kaur

Journal:

Microorganisms (2022)

Microvascular neural blood flow assessment for a chronic nerve compression neuropathy mouse model by fluorescein angiography.

Authors:

Hama, Shunpei and Yokoi, Takuya and Okada, Mitsuhiro and Uemura, Takuya and Takamatsu, Kiyohito and Nakamura, Hiroaki

Journal:

Neurological research (2022): 937-945

Probing cigarette smoke-induced DNA single-strand breaks and screening natural protective compounds by use of magnetic bead-based chemiluminescence.

Authors:

Chen, Hongjun and Yu, Zicheng and Cao, Zhijuan and Lau, Choiwan

Journal:

Analytical and bioanalytical chemistry (2016): 8369-8375

Three-dimensional structure, binding, and spectroscopic characteristics of the monoclonal antibody 43.1 directed to the carboxyphenyl moiety of fluorescein.

Authors:

Gayda, Susan and Longenecker, Kenton L and Judge, Russell A and Swift, Kerry M and Manoj, Sharmila and Linthicum, D Scott and Tetin, Sergey Y

Journal:

Biopolymers (2016): 234-43

Physical properties

Solvent

Water

Antibody properties

Host	Rabbit
Application	IF, ELISA, Flow Cytometry (FACS)

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Fax
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Bulk request	Inquire
Custom size	Inquire
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Page updated on July 15, 2026