![Indirect ELISA of Rabbit Anti-ICG [Indocyanine Green] Antibody. Microplates were coated with 100 ng/well of BSA-ICG or BSA (control). Coating, blocking, and washing steps followed standard ELISA procedures. Rabbit Anti-ICG [Indocyanine Green] Antibody (Cat# 46820) was serially diluted threefold from 100 ng/well and incubated for 40 minutes. After washing, HRP-labeled goat anti-rabbit IgG (Cat# 16793) (100 ng/mL in PBST; 100 µL/well) was added and incubated at room temperature for 40 minutes. Bound HRP activity was detected using ReadiUse™ TMB Substrate Solution (Cat# 11012), and absorbance was measured at 450 nm.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Frabbit-anti-icg-indocyanine-green-antibody%2Ffigure-for-rabbit-anti-icg-indocyanine-green-antibody_3gqzy.jpeg&w=1920&q=100)
![Indirect ELISA of Rabbit Anti-ICG [Indocyanine Green] Antibody. Microplates were coated with 100 ng/well of BSA-ICG or BSA (control). Coating, blocking, and washing steps followed standard ELISA procedures. Rabbit Anti-ICG [Indocyanine Green] Antibody (Cat# 46820) was serially diluted threefold from 100 ng/well and incubated for 40 minutes. After washing, HRP-labeled goat anti-rabbit IgG (Cat# 16793) (100 ng/mL in PBST; 100 µL/well) was added and incubated at room temperature for 40 minutes. Bound HRP activity was detected using ReadiUse™ TMB Substrate Solution (Cat# 11012), and absorbance was measured at 450 nm.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Frabbit-anti-icg-indocyanine-green-antibody%2Ffigure-for-rabbit-anti-icg-indocyanine-green-antibody_3gqzy.jpeg&w=1920&q=100)
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 46820 | 1 mg | Price |
| Solvent | Water |
| Host | Rabbit |
| Application | IF, ELISA, Flow Cytometry (FACS) |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |