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ReadiLeave™ Reversible Biotin Amine

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Physical properties
Molecular weight588.68
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
588.68
ReadiLeave™ Reversible (RLR) Biotin is a newly developed biotin derivative that has significantly reduced affinity to avidin (including streptavidin) to make the binding of RLR biotin and streptavidin readily reversible when needed. It is complimentary to the regular biotin and has a moderate affinity to streptavidin to ensure a tight binding but not too tight to be reversed in contrast with the regular non-reversible biotin. ReadiLeave™ Reversible Biotin Amine is an excellent building block to develop reversible biotin probes and products for biological detections and purification using the well-known click reactions (CuAAC). It readily reacts with a carbonyl-containing biomolecule under various conditions. The affinity between streptavidin and biotin might be the strongest non-covalent interactions known in biological interactions. Streptavidin, a homotetrameric protein, exhibits an extraordinarily high affinity for biotin. Each streptavidin monomer can bind one biotin molecule, allowing a streptavidin protein to maximally bind four biotins. The streptavidin-biotin interaction is highly specific and remains robust under a wide range of conditions.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiLeave™ Reversible Biotin Amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM169.872 µL849.358 µL1.699 mL8.494 mL16.987 mL
5 mM33.974 µL169.872 µL339.743 µL1.699 mL3.397 mL
10 mM16.987 µL84.936 µL169.872 µL849.358 µL1.699 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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References


View all 50 references: Citation Explorer
Synthesis of highly fluorescent, amine-functionalized carbon dots from biotin-modified chitosan and silk-fibroin blend for target-specific delivery of antitumor agents.
Authors: Horo, Himali and Saha, Muktashree and Das, Himadree and Mandal, Bishnupada and Kundu, Lal Mohan
Journal: Carbohydrate polymers (2022): 118862
Transfer hydrogenations catalyzed by streptavidin-hosted secondary amine organocatalysts.
Authors: Santi, Nicolò and Morrill, Louis C and Swiderek, Katarzyna and Moliner, Vicent and Luk, Louis Y P
Journal: Chemical communications (Cambridge, England) (2021): 1919-1922
A brand-new generation of fluorescent nano-neural tracers: biotinylated dextran amine conjugated carbonized polymer dots.
Authors: Liu, Yang and Liu, Junjun and Zhang, Jiayi and Li, Xiucun and Lin, Fangsiyu and Zhou, Nan and Yang, Bai and Lu, Laijin
Journal: Biomaterials science (2019): 1574-1583
Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat.
Authors: Xu, Dongsheng and Cui, Jingjing and Wang, Jia and Zhang, Zhiyun and She, Chen and Bai, Wanzhu
Journal: Journal of visualized experiments : JoVE (2018)
Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction.
Authors: Jacobsen, Michael T and Fairhead, Michael and Fogelstrand, Per and Howarth, Mark
Journal: Cell chemical biology (2017): 1040-1047.e4
Anterograde and retrograde tracing with high molecular weight biotinylated dextran amine through thalamocortical and corticothalamic pathways.
Authors: Zhang, Wenjie and Xu, Dongsheng and Cui, Jingjing and Jing, Xianghong and Xu, Nenggui and Liu, Jianhua and Bai, Wanzhu
Journal: Microscopy research and technique (2017): 260-266
Amine coupling versus biotin capture for the assessment of sulfonamide as ligands of hCA isoforms.
Authors: Rogez-Florent, Tiphaine and Goossens, Laurence and Drucbert, Anne-Sophie and Duban-Deweer, Sophie and Six, Perrine and Depreux, Patrick and Danzé, Pierre-Marie and Goossens, Jean-François and Foulon, Catherine
Journal: Analytical biochemistry (2016): 42-51
A feasible approach to evaluate the relative reactivity of NHS-ester activated group with primary amine-derivatized DNA analogue and non-derivatized impurity.
Authors: Dou, Shuping and Virostko, John and Greiner, Dale L and Powers, Alvin C and Liu, Guozheng
Journal: Nucleosides, nucleotides & nucleic acids (2015): 69-78
Systematic comparison of adeno-associated virus and biotinylated dextran amine reveals equivalent sensitivity between tracers and novel projection targets in the mouse brain.
Authors: Wang, Quanxin and Henry, Alex M and Harris, Julie A and Oh, Seung Wook and Joines, Kevin M and Nyhus, Julie and Hirokawa, Karla E and Dee, Nick and Mortrud, Marty and Parry, Sheana and Ouellette, Benjamin and Caldejon, Shiella and Bernard, Amy and Jones, Allan R and Zeng, Hongkui and Hohmann, John G
Journal: The Journal of comparative neurology (2014): 1989-2012
Unexpected biotinylation using ATP-γ-Biotin-LC-PEO-amine as a kinase substrate.
Authors: Arora, Dhruv P and Boon, Elizabeth M
Journal: Biochemical and biophysical research communications (2013): 287-90