ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labelling Kit enables simple and uniform tagging of single-stranded DNA or oligos with DIG tag. The labelling kit uses our proprietary TAQuest™ terminal deoxynucleotidyl transferase (TdT) to catalyze non-template directed nucleotide incorporation onto the 3’- end of single-stranded DNAs or oligos. The kit is optimized for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos. The resulting DIG-labeled oligo and ssDNA probes are ideally suited for a variety of biological applications, e.g., electrophoretic mobility shift assays (EMSA), Northern and Southern blots, colony or in situ hybridizations.
Platform
Thermal Cycler
Instrument specification(s) | 0.5 mL microcentrifuge or 0.2 mL PCR tube |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare oligo or ssDNA samples
- Add reagents to tube
- Mix and centrifuge briefly
- Incubate at 37 °C for 60 minutes
- Place on ice for 5 minutes
- Purify the labeled DNA
Note: Thaw all the kit components on ice before starting the experiment. Briefly centrifuge all the reagents to the bottom before starting the labeling process.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be used as a guideline.
Note: The amount of DIG-dUTP can be optimized to achieve the best labeling conditions.
- To a clean (Nuclease-free) 0.5 mL microcentrifuge tube or 0.2 mL PCR tube, prepare a reaction mix by adding the reagents in the order indicated in Table 1.
- Carefully mix the reagents by a brief vortex, followed by a brief centrifuge.
- Incubate the reaction at 37 °C for 60 minutes.
- After incubation, place the reaction on ice for 5 minutes.
- Purify the labeled DNA.
Components | Amount |
Oligo or ssDNA sample | 1 µg DNA diluted in Nuclease-free water to a final volume of 5 µL |
TdT Reaction Buffer | 40 µL |
DIG-dUTP | 1-2 µL |
CoCl2 | 5 µL |
TdT enzyme | 0.5 µL |
Total Volume | 52 µL (Approx.) |
Images

Figure 1. Verification of single stranded DNA labeling with ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit. Starting material without DIG labeling (-) and starting material with DIG labeling (+) were run on 4% agarose gel followed by gel staining with SYBR™ Gold Nucleic Acid Gel Stain. DIG labeled ssDNA has more bases due to labeling, thus shows higher band pattern compared to control.
Citations
View all 1 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
References
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Authors: Ahmad, Waqar and Gull, Bushra and Baby, Jasmin and Mustafa, Farah
Journal: Current issues in molecular biology (2021): 457-484
Authors: Ahmad, Waqar and Gull, Bushra and Baby, Jasmin and Mustafa, Farah
Journal: Current issues in molecular biology (2021): 457-484
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Authors: Konstantou, Jessica and Ioannou, Penelope C and Christopoulos, Theodore K
Journal: Analytical and bioanalytical chemistry (2007): 1747-54
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