ReadiUse™ NADPH Regenerating Kit

ReadiUse™ NADPH Regenerating Kit provides a convenient system for regenerating NADPH in enzyme assays requiring a continuous supply of reducing equivalents.

NADPH Regeneration: Maintains NADPH availability for enzyme-catalyzed reactions.
Enzyme-Based Chemistry: Uses glucose-6-phosphate dehydrogenase for NADPH regeneration.
Simple Workflow: Prepare a 2X regenerating solution by mixing kit components.
Assay Capacity: Supports approximately 300–500 enzyme assays, depending on experimental design.
Applications: Suitable for P450, FMO, and other NADPH-dependent oxidase assays.

Chemical structure for ReadiUse™ NADPH Regenerating Kit

Protocol

SDS

Catalog	Size	Price	Quantity
15265	1000 Tests	Price

Overview

NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species). NADPH is also used for anabolic pathways, such as lipid synthesis, cholesterol synthesis and fatty acid chain elongation. It is the source of reducing equivalents for cytochrome 450 hydroxylation of aromatic compounds, steroids, alcohols, and drugs. NADPH is a necessary cofactor in many xenobiotic metabolism reactions. In chloroplasts, NADP is reduced by ferredoxin-NADP reductase in last step of the electron chain of the light reactions of photosynthesis. The NADPH produced is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. Many oxidoreductases and all ligases use NADPH as coenzymes. NADPH is required for the measurement of oxidase activity catalyzed by P450s, FMOs, NADPH-P450 reductase, and many other oxidase enzymes. AAT Bioquest's RediUse™ NADPH Regenerating Kit provides two ready-to-use solutions to regenerate NADPH by a simple mixing. This kit can be used for all NADPH-requiring oxidase assays (cDNA-expressed enzymes and liver fractions). About 300-500 enzyme assays can be performed using this kit. The total number of assays that can be performed depends on a researcher's experimental design.

Citations

View all 8 citations: Citation Explorer

Identification of osalmid metabolic profile and active metabolites with anti-tumor activity in human hepatocellular carcinoma cells

Authors:

Wu, Zhe and Zhan, Yaqiong and Wang, Li and Tong, Jiepeng and Zhang, Li and Lin, Mengjia and Jin, Xuehang and Jiang, Lushun and Lou, Yan and Qiu, Yunqing

Journal:

Biomedicine \& Pharmacotherapy (2020): 110556

Specialized Enzymes in Insect Lipid Metabolism

Authors:

MacLean, Marina Ann

Functional characterization of CYP4G11–a highly conserved enzyme in the western honey bee Apis mellifera

Authors:

Calla, Bernarda and MacLean, Marina and Liao, Ling-Hsiu and Dhanjal, Inderpreet and Tittiger, Claus and Blomquist, Gary J and Berenbaum, May R

Journal:

Insect molecular biology (2018)

Immunotoxic effects of benzo [a] pyrene on rainbow trout (Oncorhynchus mykiss)

Authors:

Phalen, Laura Jane

exo-Brevicomin biosynthetic pathway enzymes from the Mountain Pine Beetle, Dendroctonus ponderosae

Authors:

Song, Minmin and Delaplain, Patrick and Nguyen, Trang T and Liu, Xibei and Wickenberg, Leah and Jeffrey, Christopher and Blomquist, Gary J and Tittiger, Claus

Journal:

Insect biochemistry and molecular biology (2014): 73--80

References

View all 14 references: Citation Explorer

High-level expression of recombinant glucose dehydrogenase and its application in NADPH regeneration

Authors:

Xu Z, Jing K, Liu Y, Cen P.

Journal:

J Ind Microbiol Biotechnol (2007): 83

Changes in NADPH diaphorase expression in the fish visual system during optic nerve regeneration and retinal development

Authors:

Devadas M, Liu Z, Kaneda M, Arai K, Matsukawa T, Kato S.

Journal:

Neurosci Res (2001): 359

Improved assay of hepatic microsomal cholesterol 7 alpha-hydroxylase activity by the use of hydroxypropyl-beta-cyclodextrin and an NADPH-regenerating system

Authors:

Souidi M, Parquet M, Lutton C.

Journal:

Clin Chim Acta (1998): 201

Cellular antioxidant defense by a ubiquinol-regenerating system coupled with cytosolic NADPH-dependent ubiquinone reductase: protective effect against carbon tetrachloride-induced hepatotoxicity in the rat

Authors:

Takahashi T, Sugimoto N, Takahata K, Okamoto T, Kishi T.

Journal:

Biol Pharm Bull (1996): 1005

Microsomal retinal synthesis: retinol vs. holo-CRBP as substrate and evaluation of NADP, NAD and NADPH as cofactors

Authors:

Napoli JL, Posch KC, Burns RD.

Journal:

Biochim Biophys Acta (1992): 183

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 28, 2026

Component A: Assay Buffer I	1 bottle (25 mL)
Component B: Assay Buffer II	1 bottle (25 mL)
Component C: 500X Glucose-6-phosphate dehydrogenase (400 units/mL)	1 vial (100 µL)