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ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*

HRP dose response was measured with Amplite® ADHP (cat#11000) and ReadiUse™ hydrogen peroxide solution (cat#11004).
HRP dose response was measured with Amplite® ADHP (cat#11000) and ReadiUse™ hydrogen peroxide solution (cat#11004).
HRP dose response was measured with Amplite® ADHP (cat#11000) and ReadiUse™ hydrogen peroxide solution (cat#11004).
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Physical properties
Molecular weight34.01
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Compared to other commercial hydrogen peroxide solutions, our formulated hydrogen peroxide solution is much more stable. It is calibrated to ensure more reproducible peroxidase-based assays.

Example protocol


Important notes
The following procedure can be adapted for most perioxidase and hydrogen peroxide based assays. In initial experiments, it may be best to try several hydrogen peroxide solutions over the entire suggested range to determine the concentration that yields optimal perioxidase.


Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. Peroxidase reaction mixture:
Prepare a Peroxidase reaction mixture with an appropriate hydrogen peroxide substrate concentration and 50 to 500 µM hydrogen peroxide.


  1. Add equal volume of Peroxidase working solution and peroxidase standards and peroxidase-containing samples.

  2. Incubate the reaction for 15 to 30 minutes at room temperature, protected from light.

  3. Monitor the fluorescence or absorbance with appropriate instruments.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM2.94 mL14.702 mL29.403 mL147.016 mL294.031 mL
5 mM588.062 µL2.94 mL5.881 mL29.403 mL58.806 mL
10 mM294.031 µL1.47 mL2.94 mL14.702 mL29.403 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 55 references: Citation Explorer
Development of enzyme-based bar code-style lateral-flow assay for hydrogen peroxide determination
Authors: Fung KK, Chan CP, Renneberg R.
Journal: Anal Chim Acta (2009): 89
The accuracy of Amplex Red assay for hydrogen peroxide in the presence of nanoparticles
Authors: Lee CW, Chen YC, Ostafin A.
Journal: J Biomed Nanotechnol (2009): 477
Development of a 384-well colorimetric assay to quantify hydrogen peroxide generated by the redox cycling of compounds in the presence of reducing agents
Authors: Johnston PA, Soares KM, Shinde SN, Foster CA, Shun TY, Takyi HK, Wipf P, Lazo JS.
Journal: Assay Drug Dev Technol (2008): 505
Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation
Authors: Ozyurek M, Bektasoglu B, Guclu K, Apak R.
Journal: Anal Chim Acta (2008): 196
Homogeneous, unmodified gold nanoparticle-based colorimetric assay of hydrogen peroxide
Authors: Wu ZS, Zhang SB, Guo MM, Chen CR, Shen GL, Yu RQ.
Journal: Anal Chim Acta (2007): 122
A reporter system for the individual detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo
Authors: Shao N, Krieger-Liszkay A, Schroda M, Beck CF.
Journal: Plant J (2007): 475
Polypyrrole-based optical probe for a hydrogen peroxide assay
Authors: Wang H, Park SM.
Journal: Anal Chem (2007): 240
Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol-microperoxidase in the presence of 1-methoxy PMS
Authors: Arakawa H, Masuda K, Tajima N, Maeda M.
Journal: Luminescence (2007): 245
The responses of lymphocytes from Asian and Caucasian diabetic patients and non-diabetics to hydrogen peroxide and sodium nitrite in the Comet assay
Authors: Wyatt N, Kelly C, Fontana V, Merlo DF, Whitelaw D, Anderson D.
Journal: Mutat Res (2006): 154
Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b6f complex: a simple and effective assay for stability of the complex in detergent solutions
Authors: Chen XB, Zhao XH, Zhu Y, Gong YD, Li LB, Zhang JP, Kuang TY.
Journal: Photosynth Res (2006): 205