ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 34.01 |
| Solvent | Water |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Buffers and Lab Consumables
Molecular weight 34.01 |
Compared to other commercial hydrogen peroxide solutions, our formulated hydrogen peroxide solution is much more stable. It is calibrated to ensure more reproducible peroxidase-based assays.
Example protocol
AT A GLANCE
Important notes
The following procedure can be adapted for most perioxidase and hydrogen peroxide based assays. In initial experiments, it may be best to try several hydrogen peroxide solutions over the entire suggested range to determine the concentration that yields optimal perioxidase.
PREPARATION OF STOCK SOLUTION
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1. Peroxidase reaction mixture:
Prepare a Peroxidase reaction mixture with an appropriate hydrogen peroxide substrate concentration and 50 to 500 µM hydrogen peroxide.
SAMPLE EXPERIMENTAL PROTOCOL
- Add equal volume of Peroxidase working solution and peroxidase standards and peroxidase-containing samples.
- Incubate the reaction for 15 to 30 minutes at room temperature, protected from light.
- Monitor the fluorescence or absorbance with appropriate instruments.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.94 mL | 14.702 mL | 29.403 mL | 147.016 mL | 294.031 mL |
| 5 mM | 588.062 µL | 2.94 mL | 5.881 mL | 29.403 mL | 58.806 mL |
| 10 mM | 294.031 µL | 1.47 mL | 2.94 mL | 14.702 mL | 29.403 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. HRP dose response was measured with Amplite® ADHP (cat#11000) and ReadiUse™ hydrogen peroxide solution (cat#11004).
References
View all 55 references: Citation Explorer
Development of enzyme-based bar code-style lateral-flow assay for hydrogen peroxide determination
Authors: Fung KK, Chan CP, Renneberg R.
Journal: Anal Chim Acta (2009): 89
Authors: Fung KK, Chan CP, Renneberg R.
Journal: Anal Chim Acta (2009): 89
The accuracy of Amplex Red assay for hydrogen peroxide in the presence of nanoparticles
Authors: Lee CW, Chen YC, Ostafin A.
Journal: J Biomed Nanotechnol (2009): 477
Authors: Lee CW, Chen YC, Ostafin A.
Journal: J Biomed Nanotechnol (2009): 477
Development of a 384-well colorimetric assay to quantify hydrogen peroxide generated by the redox cycling of compounds in the presence of reducing agents
Authors: Johnston PA, Soares KM, Shinde SN, Foster CA, Shun TY, Takyi HK, Wipf P, Lazo JS.
Journal: Assay Drug Dev Technol (2008): 505
Authors: Johnston PA, Soares KM, Shinde SN, Foster CA, Shun TY, Takyi HK, Wipf P, Lazo JS.
Journal: Assay Drug Dev Technol (2008): 505
Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation
Authors: Ozyurek M, Bektasoglu B, Guclu K, Apak R.
Journal: Anal Chim Acta (2008): 196
Authors: Ozyurek M, Bektasoglu B, Guclu K, Apak R.
Journal: Anal Chim Acta (2008): 196
Homogeneous, unmodified gold nanoparticle-based colorimetric assay of hydrogen peroxide
Authors: Wu ZS, Zhang SB, Guo MM, Chen CR, Shen GL, Yu RQ.
Journal: Anal Chim Acta (2007): 122
Authors: Wu ZS, Zhang SB, Guo MM, Chen CR, Shen GL, Yu RQ.
Journal: Anal Chim Acta (2007): 122
A reporter system for the individual detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo
Authors: Shao N, Krieger-Liszkay A, Schroda M, Beck CF.
Journal: Plant J (2007): 475
Authors: Shao N, Krieger-Liszkay A, Schroda M, Beck CF.
Journal: Plant J (2007): 475
Polypyrrole-based optical probe for a hydrogen peroxide assay
Authors: Wang H, Park SM.
Journal: Anal Chem (2007): 240
Authors: Wang H, Park SM.
Journal: Anal Chem (2007): 240
Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol-microperoxidase in the presence of 1-methoxy PMS
Authors: Arakawa H, Masuda K, Tajima N, Maeda M.
Journal: Luminescence (2007): 245
Authors: Arakawa H, Masuda K, Tajima N, Maeda M.
Journal: Luminescence (2007): 245
The responses of lymphocytes from Asian and Caucasian diabetic patients and non-diabetics to hydrogen peroxide and sodium nitrite in the Comet assay
Authors: Wyatt N, Kelly C, Fontana V, Merlo DF, Whitelaw D, Anderson D.
Journal: Mutat Res (2006): 154
Authors: Wyatt N, Kelly C, Fontana V, Merlo DF, Whitelaw D, Anderson D.
Journal: Mutat Res (2006): 154
Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b6f complex: a simple and effective assay for stability of the complex in detergent solutions
Authors: Chen XB, Zhao XH, Zhu Y, Gong YD, Li LB, Zhang JP, Kuang TY.
Journal: Photosynth Res (2006): 205
Authors: Chen XB, Zhao XH, Zhu Y, Gong YD, Li LB, Zhang JP, Kuang TY.
Journal: Photosynth Res (2006): 205