ReadiView™ biotin succinimidyl ester

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The structure of ReadiView™ biotin succinimidyl ester that is imbedded with a conjugation TAG. This imbedded TAT can be easily monitored by its UV absorption distinct from the 280 nm absorption of proteins. The TAG does not quench fluorescence of the commonly used fluorescent dyes with the minimal effect on avidin binding.
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Unit Size: Cat No: Price (USD): Qty:
5 mg 3059 $95


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Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

MW703.81
SolventDMSO
Storage Freeze (<-15 °C)
Category Biotin and Streptavidin
Amine-Reactive Biotins
Related Amine-Reactive Probes
Biotin/avidin complexes are widely applied for a variety of biological detections. Although a large number of biotin-labeled bioconjugates are commercially available, the accurate determination of biotinylation degree (ratio of biotin/biopolymer) is still a great challenge for biochemists. HABA is still predominantly used for determining the degree of biotinylation (through its absorption with the extinction coefficient = 34,000/M-1cm-1). When a biotin-containing sample is added, the biotin binds strongly to avidin and displaces the weakly bound HABA. The resulting decrease in absorbance relates to the amount of biotin. However there are many factors that affect the accuracy of HABA method, making this method unreliable for many biotin-labeled conjugates. Our ReadiView™ biotin contains specially designed Color Tag (CT) that makes the biotinylation degree readily accessible by simply calculating the corrected absorption ratio of 280 nm/385 nm. Our specially designed tag has very minimal effect on the biotin binding affinity, and its absorption maximum was designed to make the tag have minimal quenching effect on the most fluorophores that are used for labeling avidins.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiView™ biotin succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 










Interactive Product Finder

Reactive forms
Product nameCurrent ProductRelated Product
ReadiView™ biotin maleimide
succinimidyl ester
maleimide
ReadiView™ biotin acid
succinimidyl ester
acid
ReadiView™ biotin hydrazide
succinimidyl ester
hydrazide
See More (1)
ReadiView™ biotin amine
succinimidyl ester
amine






References

A low-cost, high-performance system for fluorescence lateral flow assays
Authors: Linda G Lee, Eric S Nordman, Martin D Johnson, Mark F Oldham
Journal: Biosensors (2013): 360--373

Implementation of P22 viral capsids as nanoplatforms
Authors: Sebyung Kang, Masaki Uchida, Alison O’Neil, Rui Li, Peter E Prevelige, Trevor Douglas
Journal: Biomacromolecules (2010): 2804--2809

Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields
Authors: Keiko Morotomi-Yano, Ken-ichi Yano
Journal: FEBS Open Bio