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ReadiView™ biotin succinimidyl ester
Biotin/avidin complexes are widely applied for a variety of biological detections. Although a large number of biotin-labeled bioconjugates are commercially available, the accurate determination of biotinylation degree (ratio of biotin/biopolymer) is still a great challenge for biochemists. HABA is still predominantly used for determining the degree of biotinylation (through its absorption with the extinction coefficient = 34,000/M-1cm-1). When a biotin-containing sample is added, the biotin binds strongly to avidin and displaces the weakly bound HABA. The resulting decrease in absorbance relates to the amount of biotin. However there are many factors that affect the accuracy of HABA method, making this method unreliable for many biotin-labeled conjugates. Our ReadiView™ biotin contains specially designed Color Tag (CT) that makes the biotinylation degree readily accessible by simply calculating the corrected absorption ratio of 280 nm/385 nm. Our specially designed tag has very minimal effect on the biotin binding affinity, and its absorption maximum was designed to make the tag have minimal quenching effect on the most fluorophores that are used for labeling avidins.
The structure of ReadiView™ biotin succinimidyl ester that is imbedded with a conjugation TAG. This imbedded TAT can be easily monitored by its UV absorption distinct from the 280 nm absorption of proteins. The TAG does not quench fluorescence of the commonly used fluorescent dyes with the minimal effect on avidin binding.
The structure of ReadiView™ biotin succinimidyl ester that is imbedded with a conjugation TAG. This imbedded TAT can be easily monitored by its UV absorption distinct from the 280 nm absorption of proteins. The TAG does not quench fluorescence of the commonly used fluorescent dyes with the minimal effect on avidin binding.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
30595 mg
Price
 
Physical properties
Molecular weight703.81
SolventDMSO
Spectral properties
Correction factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)
15000
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C)
UNSPSC12352200
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Page updated on October 10, 2025