Name: Screen Quest™ Fluorimetric MDR Assay Kit
Brand: AAT Bioquest
SKU: 36340
Availability: InStock

Screen Quest™ Fluorimetric MDR Assay Kit

Screen Quest™ Fluorimetric MDR Assay Kit is a fluorescence-based kit for assessing MDR1- and/or MRP1-associated efflux activity through cellular retention of a fluorescent indicator in microplate assays.

Functional efflux readout: Uses a cell-permeable fluorescent MDR indicator to assess MDR1- and/or MRP1-associated dye efflux
Inhibition-responsive signal: Reduced indicator efflux increases intracellular dye retention and cellular fluorescence
Microplate compatibility: Supports 96-well and 384-well formats and automated bottom-read fluorescence detection
Research applications: Suitable for screening MDR efflux inhibitors and comparing efflux-associated dye retention across cell models

Effect of Cyclosporin A on the inhibition of P-gp pump in MCF-7/ADR cells. The increased concentration of Cyclosporin A resulted in an increase in fluorescence signal caused by the inhibition of P-gp pump which enhanced the intracellular accumulation of MDR indicator dye. The EC50 = 2.4 μM (measured with the kit) is similar to the value reported in the literature.

Protocol

COA

Catalog	Size	Price	Quantity
36340	100 Tests	Price
36341	1000 Tests	Price

Overview

Screen Quest™ Fluorimetric MDR Assay Kit assesses multidrug resistance–associated efflux using a cell-permeable fluorescent indicator. In cells expressing P-glycoprotein (Pgp/MDR1) and/or multidrug resistance–associated protein 1 (MRP1), transporter-mediated extrusion reduces intracellular indicator retention and cellular fluorescence. Inhibition of indicator efflux increases dye retention, providing a functional readout of changes in efflux activity.

The assay supports 96-well and 384-well microplates and automated bottom-read fluorescence detection, with recommended reader settings of Ex/Em = 490/525 nm. The shared indicator readout does not independently distinguish MDR1 from MRP1 activity; transporter attribution requires appropriate controls. Compound concentration and exposure time require empirical optimization for the selected cell model, with matched DMSO vehicle controls where relevant. The kit supports screening of MDR efflux inhibitors and functional studies of transporter-associated multidrug resistance.

References

View all 77 references: Citation Explorer

Mutational Patterns Associated with the 69 Insertion Complex in Multi-drug-resistant HIV-1 Reverse Transcriptase that Confer Increased Excision Activity and High-level Resistance to Zidovudine

Authors:

Cases-Gonzalez CE, Franco S, Martinez MA, Menendez-Arias L.

Journal:

J Mol Biol (2007): 298

A phase I clinical and pharmacokinetic study of the multi-drug resistance protein-1 (MRP-1) inhibitor sulindac, in combination with epirubicin in patients with advanced cancer

Authors:

O'Connor R, O'Leary M, Ballot J, Collins CD, Kinsella P, Mager DE, Arnold RD, O'Driscoll L, Larkin A, Kennedy S, Fennelly D, Clynes M, Crown J.

Journal:

Cancer Chemother Pharmacol (2007): 79

Identification of multi-drug resistant Pseudomonas aeruginosa clinical isolates that are highly disruptive to the intestinal epithelial barrier

Authors:

Zaborina O, Kohler JE, Wang Y, Bethel C, Shevchenko O, Wu L, Turner JR, Alverdy JC.

Journal:

Ann Clin Microbiol Antimicrob (2006): 14

Therapeutic significance of ectophosphatase inhibitors in reversal of multi-drug resistance

Authors:

Kannan S., undefined

Journal:

Med Hypotheses (2006): 1041

Newer diagnostics for tuberculosis and multi-drug resistant tuberculosis

Authors:

Palomino JC., undefined

Journal:

Curr Opin Pulm Med (2006): 172

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

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Page updated on October 8, 2026

Component A: MDR Sensor	1 vial, lyophilized
Component B: DMSO	1 vial (100 µL)
Component C: Assay Buffer	1 bottle (10 mL)

Component A: MDR Sensor	1 vial, lyophilized
Component B: DMSO	1 vial (300 µL)
Component C: Assay Buffer	1 bottle (100 mL)