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Screen Quest™ Fura-2 No Wash Calcium Assay Kit
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This ratiometric calcium assay kit allows homogeneous measurement of intracellular calcium changes caused by activation of G-protein-coupled receptors or calcium channels. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fura-2 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Fura-2 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells and its fluorescence wavelength is blue-shifted upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which greatly increase the fluorescence intensity of Fura-2 at the short wavelength. The ratiometric characteristics of Fura-2 make this kit an ideal tool for more accurate measurement of cellular calcium concentration compared to Fluo-4 of the single wavelength. With a single addition, the assay is easy to perform and desirable in a high thoroughput environment. The assay can be used in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation.
ATP dose response in CHO-K1 cells measured with Screen Quest™ Fura-2 No Wash Calcium Assay Kit. CHO-K1 cells were seeded overnight at 40,000 cells/100 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of Screen Quest™ Fura-2 No Wash Calcium Assay Kit for 1 hour at room temperature. ATP (50 µL/well) was added by a FlexStation (Molecular Devices) to achieve the final indicated concentrations.
ATP dose response in CHO-K1 cells measured with Screen Quest™ Fura-2 No Wash Calcium Assay Kit. CHO-K1 cells were seeded overnight at 40,000 cells/100 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL of Screen Quest™ Fura-2 No Wash Calcium Assay Kit for 1 hour at room temperature. ATP (50 µL/well) was added by a FlexStation (Molecular Devices) to achieve the final indicated concentrations.
protocol
Protocol
COA
CatalogSize
Price
Quantity
3632010 Plates
Price
36321100 Plates
Price
 
Spectral properties
Excitation (nm)336
Emission (nm)505
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation340/380 nm
Emission510 nm
Cutoff470 nm
Recommended plateBlack wall/Clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
Other instrumentsFDSS, FLIPR, FlexStation
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Page updated on October 13, 2025