TAQuest™ qPCR Master Mix with Helixyte™ Green High ROX

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

Overview SDS Protocol

TAQuest™ qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. The master mix includes our proprietary TAQuest™ Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer. You only need to add template and target primers to run the desired PCR reactions. The Hot Start Taq DNA polymerase allows you to set up a PCR reaction at room temperature, thus minimizing non-specific product formation. In combination with an optimized buffer, the enzyme ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. The Helixyte Green intercalating dye allows rapid DNA detection and analysis without using sequence-specific probes. This master mix contains a high amount of ROX reference dye.

Platform

qPCR

Instrument specification(s)

SYBR Green filter

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline.
Note Thaw the TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* at room temperature. Vortex qPCR Master Mix thoroughly before use.

Prepare one of the following reaction mixes as indicated in Table 1.
Carefully mix the reagents with a gentle vortex followed by a brief centrifuge.
Set up the plate in the qPCR instrument and run as indicated in Table 2.

Table 1.Reagents composition per well for each reaction

Components	Volume (25 µL/reaction)	Volume (50 µL/reaction)	Final Conc.
TAQuest™ qPCR Master Mix with Helixyte™ Green High ROX	12.5 µL	25 µL	1X
Upstream primer, 10 µM	0.25-2.5 µL	0.5-5.0 µL	0.1-1.0 µM
Downstream primer, 10 µM	0.25-2.5 µL	0.5-5.0 µL	0.1-1.0 µM
DNA template	1-5 µL	1-5 µL	Optimized conc.
Nuclease-Free Water to	25 µL	50 µL

Table 2.Thermal cycling parameters

Parameter	Polymerase Activation	PCR (30-40 cycles)
	Hold	Denature	Anneal	Extend
Temperature	95 °C	95 °C	55-65 °C	68-72 °C
Time (m:ss)	0:20	0:30	1:00	1:00

References

View all 7 references: Citation Explorer

Aligned Expression of IFI16 and STING Genes in RRMS Patients' Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32

Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.
Authors: Siddiqi, Allauddin and Milne, Trudy and Cullinan, Mary P and Seymour, Gregory J
Journal: Clinical oral implants research (2016): 288-94

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199

Real-time immuno-PCR for ultrasensitive detection of pyrene and other homologous PAHs.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Zhang, Y Y and Qiao, B and Sun, Y and Yang, L and Hu, P and Lu, S Y and Ren, H L and Zhang, J H and Wang, X R and Liu, Z S
Journal: Biosensors & bioelectronics (2015): 42-7

Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.
Authors: Ramos, Carlos A N and Araújo, Flábio R and Souza, Ingrid I F and Bacanelli, G and Luiz, Hera L and Russi, Lívia S and Oliveira, Renato H M and Soares, Cleber O and Rosinha, Grácia M S and Alves, Leucio C
Journal: Veterinary parasitology (2011): 79-83