AAT Bioquest

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*.
Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*.
Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*.
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Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


TAQuest™ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. The master mix is compatible with FAST conditions, thus delivers results within 50 minutes for 40 cycles of PCR in a 20 uL reaction volume. The master mix provides all of the essential components including our proprietary TAQuest™ FAST Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer, except the template, primers and probes. TAQuest™ FAST qPCR Master Mix for TaqMan Probes has been designed to be used for duplex reactions using internal positive controls with superior performance. The master mix ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. This master mix contains a low amount of ROX reference dye.



Instrument specification(s)Filter based on probes

Example protocol


The following protocol can be used as a guideline.
Note     Thaw the TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* at room temperature. Vortex qPCR Master Mix thoroughly before use.
  1. Prepare one of the following reaction mixes as indicated in Table 1.
  2. Carefully mix the reagents with a gentle vortex followed by a brief centrifuge.
  3. Set up the plate in the qPCR instrument and run as indicated in Table 2. 
Table 1.Reagents composition per well for each reaction
ComponentsVolume (25 µL/reaction)Volume (50 µL/reaction)Final Conc.
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*12.5 µL25 µL1X
Upstream primer, 10 µM0.25-2.5 µL0.5-5.0 µL0.1-1.0 µM
Downstream primer, 10 µM0.25-2.5 µL0.5-5.0 µL0.1-1.0 µM
TaqMan Probes, 10 µM0.25-0.625 µL0.5-1.25 µL100-250 nM
DNA template1-5 µL1-5 µLOptimized conc.
Nuclease-Free Water to25 µL50 µL 
Table 2.Thermal cycling parameters
Parameter Polymerase Activation PCR (30-40 cycles)
  Hold Denature Anneal/Extend
Temperature 95 °C 95 °C 60 °C
Time (m:ss) 0:10 0:20 0:30



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BlueTYPE - A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
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Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
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