TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

TAQuest™ FAST qPCR Master Mix for TaqMan Probes

Low ROX

TAQuest™ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. The master mix is compatible with FAST conditions, thus delivers results within 50 minutes for 40 cycles of PCR in a 20 uL reaction volume. The master mix provides all of the essential components including our proprietary TAQuest™ FAST Hot Start Taq DNA Polymerase enzyme and dNTPs in an optimized PCR buffer, except the template, primers and probes. TAQuest™ FAST qPCR Master Mix for TaqMan Probes has been designed to be used for duplex reactions using internal positive controls with superior performance. The master mix ensures PCR specificity and sensitivity with all sample types such as genomic, plasmid, viral and cDNA templates. This master mix contains a low amount of ROX reference dye.

Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*.

Protocol

COA

Catalog	Size	Price	Quantity
17290	1 mL	Price
17291	5 mL	Price

References

View all 50 references: Citation Explorer

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.

Authors:

Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim

Journal:

Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.

Authors:

Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V

Journal:

Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).

Authors:

Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio

Journal:

Journal of economic entomology (2021): 90-99

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.

Authors:

Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian

Journal:

Human cell (2020): 1331-1333

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.

Authors:

Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo

Journal:

Molecular and cellular probes (2020): 101618

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

qPCR
Instrument specification(s)	Filter based on probes

Documents

Protocol
Safety Data Sheet (Catalog 17290)
Safety Data Sheet (Catalog 17291)
Certificate of Analysis

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Page updated on October 23, 2025