TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

TAQuest™ FAST qPCR Master Mix for TaqMan Probes

Low ROX

TAQuest™ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X probe-based qPCR master mix with low ROX reference dye for fast qPCR and 2-step RT-qPCR workflows.

Ready-to-use 2X master mix: Provides TAQuest™ FAST Hot Start Taq DNA Polymerase, dNTPs, optimized PCR buffer, and low ROX reference dye
FAST cycling compatibility: Supports fast qPCR cycling, with 40 PCR cycles completed in approximately 50 minutes using a 20 µL reaction volume
TaqMan probe format: Optimized for probe-based qPCR workflows, including TaqMan gene expression assays
Duplex assay support: Designed for duplex reactions using internal positive controls
Research applications: Suitable for qPCR and 2-step RT-qPCR using genomic DNA, plasmid DNA, viral templates, or cDNA

Amplification plot for a dilution series of HeLa cells cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*.

Protocol

COA

Catalog	Size	Price	Quantity
17290	1 mL	Price
17291	5 mL	Price

Overview

TAQuest™ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for real-time PCR and 2-step RT-qPCR using probe-based detection. The master mix contains TAQuest™ FAST Hot Start Taq DNA Polymerase, dNTPs, an optimized PCR buffer, and low ROX reference dye. Template, primers, and probes are added separately to prepare the desired qPCR reactions.

TAQuest™ FAST qPCR Master Mix for TaqMan Probes is compatible with FAST cycling conditions and supports completion of 40 PCR cycles in approximately 50 minutes using a 20 µL reaction volume. The TAQuest™ FAST Hot Start Taq DNA Polymerase is designed to support specific and sensitive amplification by reducing non-specific product formation during reaction setup. The formulation is also designed for duplex reactions using internal positive controls and supports amplification from genomic DNA, plasmid DNA, viral templates, and cDNA. TAQuest™ FAST qPCR Master Mix for TaqMan Probes (Low ROX) is suitable for TaqMan gene expression assays, probe-based qPCR, duplex qPCR, and 2-step RT-qPCR applications.

References

View all 50 references: Citation Explorer

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.

Authors:

Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim

Journal:

Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.

Authors:

Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V

Journal:

Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).

Authors:

Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio

Journal:

Journal of economic entomology (2021): 90-99

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.

Authors:

Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian

Journal:

Human cell (2020): 1331-1333

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.

Authors:

Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo

Journal:

Molecular and cellular probes (2020): 101618

Physical properties

Solvent

Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

qPCR
Instrument specification(s)	Filter based on probes

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Page updated on September 7, 2026