Name: TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
Brand: AAT Bioquest
SKU: 17280
Availability: InStock

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green

High ROX

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X dye-based qPCR master mix with high ROX reference dye for fast qPCR and 2-step RT-qPCR workflows.

Ready-to-use 2X master mix: Contains TAQuest™ FAST Hot Start Taq DNA Polymerase, dNTPs, optimized PCR buffer, Helixyte™ Green dye, and high ROX reference dye
FAST cycling workflow: Supports completion of 40 PCR cycles in approximately 50 minutes using a 20 µL reaction volume
Instant hot start enzyme: Designed to minimize non-specific product formation and support room-temperature reaction setup
Probe-free detection: Uses Helixyte™ Green intercalating dye for real-time DNA detection without sequence-specific probes
Research applications: Suitable for qPCR and 2-step RT-qPCR using genomic DNA, plasmid DNA, viral templates, or cDNA

General packaging for catalogs 17280 and 17281. Actual shipped packaging may vary.

Protocol

COA

Catalog	Size	Price	Quantity
17280	1 mL	Price
17281	5 mL	Price

Overview

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green is a ready-to-use 2X solution optimized for real-time PCR and 2-step RT-qPCR. The master mix contains an optimized PCR buffer, dNTPs, Helixyte™ Green intercalating dye, high ROX reference dye, and proprietary TAQuest™ FAST Hot Start Taq DNA Polymerase. Only template and target-specific primers are required to prepare the desired qPCR reactions.

TAQuest™ FAST Hot Start Taq DNA Polymerase is designed to provide instant hot start activity, helping minimize non-specific product formation while allowing room-temperature reaction setup. The formulation supports fast qPCR cycling and delivers results within approximately 50 minutes for 40 cycles in a 20 µL reaction volume. Helixyte™ Green enables rapid DNA detection and analysis without the need for sequence-specific probes, while the high ROX reference dye supports instruments that require high ROX normalization. TAQuest™ FAST qPCR Master Mix with Helixyte™ Green (High ROX) is suitable for dye-based qPCR and 2-step RT-qPCR analysis of genomic DNA, plasmid DNA, viral templates, and cDNA samples.

References

View all 4 references: Citation Explorer

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.

Authors:

Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O'Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa

Journal:

PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.

Authors:

Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin

Journal:

Journal of virological methods (2019): 58-64

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.

Authors:

Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q

Journal:

Biosensors & bioelectronics (2016): 194-199

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.

Authors:

Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R

Journal:

Journal of medical virology (2016): 115-9

Physical properties

Solvent

Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

qPCR
Instrument specification(s)	SYBR Green filter

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 27, 2026