Tetracysteine REASH acetate *2 mM DMSO Solution*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 587.40 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 570 |
Extinction coefficient (cm -1 M -1) | 650001 |
Excitation (nm) | 571 |
Emission (nm) | 584 |
Quantum yield | 0.751 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Molecular weight 587.40 | Absorbance (nm) 570 | Extinction coefficient (cm -1 M -1) 650001 | Excitation (nm) 571 | Emission (nm) 584 | Quantum yield 0.751 |
REASH acetate is a cell-permeable version of REASH, which is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other. The biarsenical labeling technology works through the high-affinity interaction of arsenic for thiols. When REASH binds to tetracysteine sequences, its biarsenical group reacts rapidly with Cys-Cys moiety and the tag become highly fluorescent in red. The biarsenical labeling reagent REASH is one of the smallest expression tags for labeling a protein that contains a six-amino acid motif with a Cys-Cys-X1-X2-Cys-Cys amino acid sequence. The most commonly used tetracysteine is the six amino acid Cys-Cys-Pro-Gly-Cys-Cys sequence. As this sequence rarely appears in endogenous proteins, incorporating the sequence into target proteins generates a small but highly specific target for protein labeling. REASH generates a strong red fluorescent signal when binding to recombinant proteins containing the tetracysteine motif Cys-Cys-Pro-Gly-Cys-Cys. It can be used for monitoring protein localization, turnover and trafficking, receptor signaling and internalization.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tetracysteine REASH acetate *2 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 170.242 µL | 851.209 µL | 1.702 mL | 8.512 mL | 17.024 mL |
5 mM | 34.048 µL | 170.242 µL | 340.483 µL | 1.702 mL | 3.405 mL |
10 mM | 17.024 µL | 85.121 µL | 170.242 µL | 851.209 µL | 1.702 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 570 |
Extinction coefficient (cm -1 M -1) | 650001 |
Excitation (nm) | 571 |
Emission (nm) | 584 |
Quantum yield | 0.751 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Tetracysteine REASH Reagent *2 mM DMSO Solution* | 571 | 584 | 650001 | 0.751 |
References
View all 4 references: Citation Explorer
Discrete Virus Factories Form in the Cytoplasm of Cells Coinfected with Two Replication-Competent Tagged Reporter Birnaviruses That Subsequently Coalesce over Time.
Authors: Campbell, Elle A and Reddy, Vishwanatha R A P and Gray, Alice G and Wells, Joanna and Simpson, Jennifer and Skinner, Michael A and Hawes, Philippa C and Broadbent, Andrew J
Journal: Journal of virology (2020)
Authors: Campbell, Elle A and Reddy, Vishwanatha R A P and Gray, Alice G and Wells, Joanna and Simpson, Jennifer and Skinner, Michael A and Hawes, Philippa C and Broadbent, Andrew J
Journal: Journal of virology (2020)
Exploration of biarsenical chemistry--challenges in protein research.
Authors: Pomorski, Adam and Krężel, Artur
Journal: Chembiochem : a European journal of chemical biology (2011): 1152-67
Authors: Pomorski, Adam and Krężel, Artur
Journal: Chembiochem : a European journal of chemical biology (2011): 1152-67
Tetracysteine-based fluorescent tags to study protein localization and trafficking in Plasmodium falciparum-infected erythrocytes.
Authors: Crivat, Georgeta and Tokumasu, Fuyuki and Sa, Juliana Martha and Hwang, Jeeseong and Wellems, Thomas E
Journal: PloS one (2011): e22975
Authors: Crivat, Georgeta and Tokumasu, Fuyuki and Sa, Juliana Martha and Hwang, Jeeseong and Wellems, Thomas E
Journal: PloS one (2011): e22975
Tetracysteine genetic tags complexed with biarsenical ligands as a tool for investigating gap junction structure and dynamics.
Authors: Sosinsky, Gina E and Gaietta, Guido M and Hand, Galen and Deerinck, Thomas J and Han, Areum and Mackey, Mason and Adams, Stephen R and Bouwer, James and Tsien, Roger Y and Ellisman, Mark H
Journal: Cell communication & adhesion : 181-6
Authors: Sosinsky, Gina E and Gaietta, Guido M and Hand, Galen and Deerinck, Thomas J and Han, Areum and Mackey, Mason and Adams, Stephen R and Bouwer, James and Tsien, Roger Y and Ellisman, Mark H
Journal: Cell communication & adhesion : 181-6