Texas Red-DHPE CAS 187099-99-6

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Physical properties

Molecular weight	1381.84
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.36
Correction Factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97¹, 0.93²

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

187099-99-6

Molecular weight

1381.84

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.36

Correction Factor (565 nm)

0.4472

Extinction coefficient (cm ^-1 M ^-1)

116000¹

Excitation (nm)

586

Emission (nm)

603

Quantum yield

0.97¹, 0.93²

Texas Red® DHPE is a phospholipid that is labeled on the head group with the bright, red-fluorescent Texas Red® dye (excitation/emission maxima ~595/615 nm). Rhodamine-labeled phospholipids have been used as tracers for membrane traffic during endocytosis and for lipid processing in hepatocytes. Texas Red DHPE is principally employed as an energy transfer acceptor from NBD, BODIPY and fluorescein lipid probes. The longer emission wavelength of the Texas Red dye provides superior separation of the donor and acceptor emission signals in resonance energy transfer microscopy. This technique has enabled visualization of ATP-dependent fusion of liposomes with the Golgi apparatus. Membrane flux during hemagglutinin-mediated cell-cell fusion has been visualized using Texas Red DHPE and the lipophilic carbocyanine DiI as membrane labels.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red-DHPE *CAS 187099-99-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	72.367 µL	361.836 µL	723.673 µL	3.618 mL	7.237 mL
5 mM	14.473 µL	72.367 µL	144.735 µL	723.673 µL	1.447 mL
10 mM	7.237 µL	36.184 µL	72.367 µL	361.836 µL	723.673 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.23
Correction Factor (280 nm)	0.36
Correction Factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97¹, 0.93²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (565 nm)
Texas Red® hydrazide Single Isomer	586	603	116000¹	0.97¹, 0.93²	0.23	0.36	0.4472
Texas Red® cadaverine Single Isomer	586	603	116000¹	0.97¹, 0.93²	0.23	0.36	0.4472
Texas Red® maleimide Single Isomer	586	603	116000¹	0.97¹, 0.93²	0.23	0.36	0.4472
Texas Red® azide Single Isomer	586	603	116000¹	0.97¹, 0.93²	0.23	0.36	0.4472
Texas Red® alkyne Single Isomer	586	603	116000¹	0.97¹, 0.93²	0.23	0.36	0.4472
Texas Red TCO Single isomer	586	603	116000¹	0.97¹, 0.93²	0.23	0.36	0.4472

Images

Figure 1. Chemical structure for Texas Red-DHPE *CAS 187099-99-6*

Citations

View all 2 citations: Citation Explorer

Interfacial Viscoelasticity and Structure of Self-Assembled Semifluorinated Alkanes on Water
Authors: Mielke, Salom{\'e}
Journal: (2019)

Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)

References

View all 27 references: Citation Explorer

Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767

Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861

Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134

Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005

Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221

Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging
Authors: Pace HP, Sherrod SD, Monson CF, Russell DH, Cremer PS.
Journal: Anal Chem (2013): 6047

Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids
Authors: Maherani B, Arab-Tehrany E, Kheirolomoom A, Geny D, Linder M.
Journal: Biochimie (2013): 2018

In vivo digestion of bovine milk fat globules: effect of processing and interfacial structural changes. I. Gastric digestion
Authors: Gallier S, Cui J, Olson TD, Rutherfurd SM, Ye A, Moughan PJ, Singh H.
Journal: Food Chem (2013): 3273

Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor
Authors: Zhang J, Li Z, Zhang H, Wang J, Liu Y, Chen G.
Journal: J Microbiol Methods (2013): 37

Formation and diffusivity characterization of supported lipid bilayers with complex lipid compositions
Authors: Simonsson L, Hook F.
Journal: Langmuir (2012): 10528