AAT Bioquest

Texas Red TCO *Single isomer*

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Physical properties
Molecular weight815.01
Spectral properties
Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.971, 0.932
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Direct upgrades
iFluor® 594 TCO


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Correction Factor (565 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.971, 0.932
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. Texas Red-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling tetrazine-modified peptides, proteins and oligos etc.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red TCO *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM122.698 µL613.489 µL1.227 mL6.135 mL12.27 mL
5 mM24.54 µL122.698 µL245.396 µL1.227 mL2.454 mL
10 mM12.27 µL61.349 µL122.698 µL613.489 µL1.227 mL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.971, 0.932

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (565 nm)
Texas Red® hydrazide *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red® cadaverine *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red® maleimide *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red® azide *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red® alkyne *Single Isomer*58660311600010.971, 0.9320.230.360.4472
Texas Red-DHPE *CAS 187099-99-6*58660311600010.971, 0.9320.230.360.4472



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Conditionally Controlling Human TLR2 Activity via Trans-Cyclooctene Caged Ligands.
Authors: van de Graaff, Michel J and Oosenbrug, Timo and Marqvorsen, Mikkel H S and Nascimento, Clarissa R and de Geus, Mark A R and Manoury, Bénédicte and Ressing, Maaike E and van Kasteren, Sander I
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Authors: Qiu, Lin and Tan, Hui and Lin, Qingyu and Si, Zhan and Mao, Wujian and Wang, Tingting and Fu, Zhequan and Cheng, Dengfeng and Shi, Hongcheng
Journal: Molecular imaging and biology (2020): 842-853
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Injectable Click Chemistry-based Bioadhesives for Accelerated Wound Closure.
Authors: Li, Shuxin and Zhou, Jun and Huang, YiHui and Roy, Joyita and Zhou, Ning and Yum, Kyungsuk and Sun, Xiankai and Tang, Liping
Journal: Acta biomaterialia (2020): 95-104
Reversible Thiol Oxidation Inhibits the Mitochondrial ATP Synthase in Xenopus Laevis Oocytes.
Authors: Cobley, James and Noble, Anna and Bessell, Rachel and Guille, Matthew and Husi, Holger
Journal: Antioxidants (Basel, Switzerland) (2020)
Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics.
Authors: de Geus, Mark A R and Maurits, Elmer and Sarris, Alexi J C and Hansen, Thomas and Kloet, Max S and Kamphorst, Kiki and Ten Hoeve, Wolter and Robillard, Marc S and Pannwitz, Andrea and Bonnet, Sylvestre A and Codée, Jeroen D C and Filippov, Dmitri V and Overkleeft, Herman S and van Kasteren, Sander I
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Antibody Pretargeting Based on Bioorthogonal Click Chemistry for Cancer Imaging and Targeted Radionuclide Therapy.
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