Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Texas Red TCO *Single isomer*

Chemical structure for Texas Red TCO *Single isomer*.
Chemical structure for Texas Red TCO *Single isomer*.
Ordering information
Price ()
Catalog Number899
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight815.01
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.931, 0.352
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Direct upgrades
iFluor® 594 TCO

OverviewpdfSDSpdfProtocol


Molecular weight
815.01
Correction Factor (260 nm)
0.23
Correction Factor (280 nm)
0.36
Correction Factor (565 nm)
0.4472
Extinction coefficient (cm -1 M -1)
1160001
Excitation (nm)
586
Emission (nm)
603
Quantum yield
0.931, 0.352
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. Texas Red-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling tetrazine-modified peptides, proteins and oligos etc.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Texas Red TCO *Single isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM122.698 µL613.489 µL1.227 mL6.135 mL12.27 mL
5 mM24.54 µL122.698 µL245.396 µL1.227 mL2.454 mL
10 mM12.27 µL61.349 µL122.698 µL613.489 µL1.227 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.23
Correction Factor (280 nm)0.36
Correction Factor (565 nm)0.4472
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)586
Emission (nm)603
Quantum yield0.931, 0.352

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (565 nm)
Texas Red® hydrazide *Single Isomer*58660311600010.931, 0.3520.230.360.4472
Texas Red® cadaverine *Single Isomer*58660311600010.931, 0.3520.230.360.4472
Texas Red® maleimide *Single Isomer*58660311600010.931, 0.3520.230.360.4472
Texas Red® azide *Single Isomer*58660311600010.931, 0.3520.230.360.4472
Texas Red® alkyne *Single Isomer*58660311600010.931, 0.3520.230.360.4472
Texas Red-DHPE *CAS 187099-99-6*58660311600010.931, 0.3520.230.360.4472

References


View all 50 references: Citation Explorer
Conditionally Controlling Human TLR2 Activity via Trans-Cyclooctene Caged Ligands.
Authors: van de Graaff, Michel J and Oosenbrug, Timo and Marqvorsen, Mikkel H S and Nascimento, Clarissa R and de Geus, Mark A R and Manoury, Bénédicte and Ressing, Maaike E and van Kasteren, Sander I
Journal: Bioconjugate chemistry (2020): 1685-1692
Cellular Delivery of Bioorthogonal Pretargeting Therapeutics in PSMA-Positive Prostate Cancer.
Authors: Hapuarachchige, Sudath and Huang, Colin T and Donnelly, Madeline C and Bařinka, Cyril and Lupold, Shawn E and Pomper, Martin G and Artemov, Dmitri
Journal: Molecular pharmaceutics (2020): 98-108
Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging.
Authors: Ko, Jina and Oh, Juhyun and Ahmed, Maaz S and Carlson, Jonathan C T and Weissleder, Ralph
Journal: Angewandte Chemie (International ed. in English) (2020): 6839-6846
Pre-targeted Imaging of Protease Activity through In Situ Assembly of Nanoparticles.
Authors: Chen, Zixin and Chen, Min and Zhou, Kaixiang and Rao, Jianghong
Journal: Angewandte Chemie (International ed. in English) (2020): 7864-7870
Antibody Pretargeting Based on Bioorthogonal Click Chemistry for Cancer Imaging and Targeted Radionuclide Therapy.
Authors: Rondon, Aurélie and Degoul, Françoise
Journal: Bioconjugate chemistry (2020): 159-173
Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics.
Authors: de Geus, Mark A R and Maurits, Elmer and Sarris, Alexi J C and Hansen, Thomas and Kloet, Max S and Kamphorst, Kiki and Ten Hoeve, Wolter and Robillard, Marc S and Pannwitz, Andrea and Bonnet, Sylvestre A and Codée, Jeroen D C and Filippov, Dmitri V and Overkleeft, Herman S and van Kasteren, Sander I
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Hybrid Imaging Agents for Pretargeting Applications Based on Fusarinine C-Proof of Concept.
Authors: Summer, Dominik and Petrik, Milos and Mayr, Sonja and Hermann, Martin and Kaeopookum, Piriya and Pfister, Joachim and Klingler, Maximilian and Rangger, Christine and Haas, Hubertus and Decristoforo, Clemens
Journal: Molecules (Basel, Switzerland) (2020)
Reversible Thiol Oxidation Inhibits the Mitochondrial ATP Synthase in Xenopus Laevis Oocytes.
Authors: Cobley, James and Noble, Anna and Bessell, Rachel and Guille, Matthew and Husi, Holger
Journal: Antioxidants (Basel, Switzerland) (2020)
Injectable Click Chemistry-based Bioadhesives for Accelerated Wound Closure.
Authors: Li, Shuxin and Zhou, Jun and Huang, YiHui and Roy, Joyita and Zhou, Ning and Yum, Kyungsuk and Sun, Xiankai and Tang, Liping
Journal: Acta biomaterialia (2020): 95-104
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)