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Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite*

Chemical structure for Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3  phosphoramidite*.
Chemical structure for Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3  phosphoramidite*.
Ordering information
Price ()
Catalog Number2274
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1045.36
SolventMeCN
Spectral properties
Absorbance (nm)560
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)560
Emission (nm)580
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Tide Fluor™ 2 maleimide [TF2 maleimide] *Superior replacement for fluorescein*
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Tide Fluor™ 3 succinimidyl ester [TF3 SE]*Superior replacement for Cy3*
Tide Fluor™ 5WS azide [TF5WS azide]
Tide Fluor™ 5WS alkyne [TF5WS alkyne]
Tide Fluor™ 5WS acid [TF5WS acid] *Superior replacement for Cy5*
Tide Fluor™ 5WS amine [TF5WS amine] *Superior replacement for Cy5*
Tide Fluor™ 5WS maleimide [TF5WS maleimide] *Superior replacement for Cy5*
Tide Fluor™ 5WS succinimidyl ester [TF5WS SE]*Superior replacement for Cy5*
Tide Fluor™ 4 acid [TF4 acid] *Superior replacement for ROX and Texas Red*
Tide Fluor™ 4 amine [TF4 amine] *Superior replacement for ROX and Texas Red*
Tide Fluor™ 4 maleimide [TF4 maleimide] *Superior replacement for ROX and Texas Red*
Tide Fluor™ 4, succinimidyl ester [TF4 SE]*Superior replacement for ROX and Texas Red*
Tide Fluor™ 6WS acid [TF6WS acid] *Superior replacement for Cy5.5*
Tide Fluor™ 6WS amine [TF6WS amine] *Superior replacement for Cy5.5*
Tide Fluor™ 6WS maleimide [TF6WS maleimide] *Superior replacement for Cy5.5*
Tide Fluor™ 6WS succinimidyl ester [TF6WS SE]*Superior replacement for Cy5.5*
Tide Fluor™ 4 azide [TF4 azide]
Tide Fluor™ 4 alkyne [TF4 alkyne]
Tide Fluor™ 6WS azide [TF6WS azide]
Tide Fluor™ 6WS alkyne [TF6WS alkyne]
Tide Fluor™ 7WS azide [TF7WS azide]
Tide Fluor™ 7WS alkyne [TF7WS alkyne]
Tide Fluor™ 8WS azide [TF8WS azide] *Near Infrared Emission*
Tide Fluor™ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission*
Tide Fluor™ 7WS acid [TF7WS acid] *Superior replacement for Cy7*
Tide Fluor™ 7WS amine [TF7WS amine] *Superior replacement for Cy7*
Tide Fluor™ 7WS maleimide [TF7WS maleimide] *Superior replacement for Cy7*
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Tide Fluor™ 8WS acid [TF8WS acid] *Near Infrared Emission*
Tide Fluor™ 8WS amine [TF8WS amine] *Near Infrared Emission*
Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*
Tide Fluor™ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission*
Tide Fluor™ 3WS maleimide [TF3WS maleimide] *Superior replacement for Cy3*
Tide Fluor™ 3WS acid [TF3WS acid] *Superior replacement for Cy3*
Tide Fluor™ 3WS succinimidyl ester [TF3WS SE] *Superior replacement for Cy3*
Tide Fluor™ 3WS amine [TF3WS amine] *Superior replacement for Cy3*
Tide Fluor™ 2WS acid [TF2WS acid] *Superior replacement for FITC*
Tide Fluor™ 2WS succinimidyl ester [TF2WS SE] *Superior replacement for FITC*
Tide Fluor™ 2WS maleimide [TF2WS Maleimide] *Superior replacement for FITC*
Tide Fluor™ 2WS Amine [TF2WS amine] *Superior replacement for FITC*
Show More (53)

OverviewpdfSDSpdfProtocol


Molecular weight
1045.36
Absorbance (nm)
560
Extinction coefficient (cm -1 M -1)
750001
Excitation (nm)
560
Emission (nm)
580
Tide Fluor™ 3 (TF3) phosphoramidite is a high-performance alternative to Cy3 phosphoramidite for the in-synthesis labeling of oligonucleotides. TF3-labeled oligonucleotides share nearly identical fluorescence spectra to those labeled with Cy3, but the significantly larger quantum yield of TF3 produces a much brighter fluorescence signal with greater photostability than Cy3. When paired with our Tide Quencher™ 3 (TQ3), TF3 can be used to produce a variety of highly-sensitive FRET peptides and nucleotides for detecting protease activity as well as molecular beacons with enhanced sensitivity and stability.

Calculators


Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of Tide Fluor™ 3 phosphoramidite [TF3 CEP] *Superior replacement to Cy3 phosphoramidite* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM95.661 µL478.304 µL956.608 µL4.783 mL9.566 mL
5 mM19.132 µL95.661 µL191.322 µL956.608 µL1.913 mL
10 mM9.566 µL47.83 µL95.661 µL478.304 µL956.608 µL

Molarity calculator

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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)560
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)560
Emission (nm)580

Citations


View all 1 citations: Citation Explorer
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035

References


View all 33 references: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
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Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
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Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
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Journal: American journal of obstetrics and gynecology (2015): 82--e1
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Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
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Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
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Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
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