Name: Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3*
Brand: AAT Bioquest
SKU: 2270
Price: 232 USD
Availability: InStock

Tide Fluor™ 3 maleimide

TF3 maleimide; Superior replacement for Cy3

Tide Fluor™ 3 (TF3) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy3. TF3-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy3. In pairing with our Tide Quencher™ 3 (TQ3), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF3 product is used for post-labeling of thiol-modified oligonucleotides and peptides that contain cysteines.

<p>Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
2270	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	153.442 µL	767.212 µL	1.534 mL	7.672 mL	15.344 mL
5 mM	30.688 µL	153.442 µL	306.885 µL	1.534 mL	3.069 mL
10 mM	15.344 µL	76.721 µL	153.442 µL	767.212 µL	1.534 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	651.71 g/mol	=	mL	x	mM	=	mmol

Citations

View all 11 citations: Citation Explorer

To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models

Authors:

Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera

Journal:

Frontiers in bioengineering and biotechnology (2020): 552035

RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein

Authors:

Pantoom, Supansa and Konstantinidis, Georgios and Voss, Stephanie and Han, Hongmei and Hofnagel, Oliver and Li, Zhiyu and Wu, Yao-Wen

Journal:

Autophagy (2020): 1--15

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation

Authors:

Perlenfein, Tyler J and Murphy, Regina M

Journal:

Journal of Biological Chemistry (2017): jbc--M117

Using a Specific RNA--Protein Interaction To Quench the Fluorescent RNA Spinach

Authors:

Roszyk, Laura and Kollenda, Sebastian and Hennig, Sven

Journal:

ACS chemical biology (2017): 2958--2964

Spatiotemporal imaging of small GTPases activity in live cells

Authors:

Voss, Stephanie and Kr{\"u}ger, Dennis M and Koch, Oliver and Wu, Yao-Wen

Journal:

Proceedings of the National Academy of Sciences (2016): 14348--14353

References

View all 25 references: Citation Explorer

Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers

Authors:

Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.

Journal:

J Med Chem (2009): 358

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe

Authors:

Andreoni A, Bondani M, Nardo L.

Journal:

Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer

Authors:

Ogawa M, Kosaka N, Choyke PL, Kobayashi H.

Journal:

Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates

Authors:

Kim GI, Kim KW, Oh MK, Sung YM.

Journal:

Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening

Authors:

Yu X, Sainz B, Jr., Uprichard SL.

Journal:

Antimicrob Agents Chemother (2009): 4311

Physical properties

Molecular weight	651.71
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.179
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	553
Emission (nm)	578
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Oligonucleotide Labeling Reagents
Brochure: Enzyme Probes & Assay Kits
Brochure: FRET Building Blocks & Probes

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2025