Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 651.71 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.179 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 554 |
Emission (nm) | 578 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
Tide Fluor™ 3 amine [TF3 amine] *Superior replacement for Cy3* |
Tide Fluor™ 3 acid [TF3 acid] *Superior replacement for Cy3* |
Related products
Overview | ![]() ![]() |
See also: Peptide and Oligonucleotide Labeling, Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, Tide Fluor Dyes, PCR Detection of Viral DNA/RNA
Molecular weight 651.71 | Correction Factor (280 nm) 0.179 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 554 | Emission (nm) 578 |
Tide Fluor™ 3 (TF3) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy3. TF3-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy3. In pairing with our Tide Quencher™ 3 (TQ3), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF3 product is used for post-labeling of thiol-modified oligonucleotides and peptides that contain cysteines.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 153.442 µL | 767.212 µL | 1.534 mL | 7.672 mL | 15.344 mL |
5 mM | 30.688 µL | 153.442 µL | 306.885 µL | 1.534 mL | 3.069 mL |
10 mM | 15.344 µL | 76.721 µL | 153.442 µL | 767.212 µL | 1.534 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (280 nm) | 0.179 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 554 |
Emission (nm) | 578 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS* | 341 | 448 | 20000 | 0.187 |
Tide Fluor™ 2 maleimide [TF2 maleimide] *Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
Tide Fluor™ 3 azide [TF3 azide] | 554 | 578 | 750001 | 0.179 |
Tide Fluor™ 3 alkyne [TF3 alkyne] | 554 | 578 | 750001 | 0.179 |
Tide Fluor™ 5WS maleimide [TF5WS maleimide] *Superior replacement for Cy5* | 649 | 664 | 250000 | 0.027 |
Tide Fluor™ 4 maleimide [TF4 maleimide] *Superior replacement for ROX and Texas Red* | 578 | 602 | 90000 | 0.436 |
Tide Fluor™ 6WS maleimide [TF6WS maleimide] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.101 |
Tide Fluor™ 7WS maleimide [TF7WS maleimide] *Superior replacement for Cy7* | 756 | 780 | 275000 | 0.049 |
Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission* | 785 | 801 | 250000 | 0.109 |
Show More (4) |
Images

Figure 1. Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Citations
View all 11 citations: Citation Explorer
RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein
Authors: Pantoom, Supansa and Konstantinidis, Georgios and Voss, Stephanie and Han, Hongmei and Hofnagel, Oliver and Li, Zhiyu and Wu, Yao-Wen
Journal: Autophagy (2020): 1--15
Authors: Pantoom, Supansa and Konstantinidis, Georgios and Voss, Stephanie and Han, Hongmei and Hofnagel, Oliver and Li, Zhiyu and Wu, Yao-Wen
Journal: Autophagy (2020): 1--15
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Using a Specific RNA--Protein Interaction To Quench the Fluorescent RNA Spinach
Authors: Roszyk, Laura and Kollenda, Sebastian and Hennig, Sven
Journal: ACS chemical biology (2017): 2958--2964
Authors: Roszyk, Laura and Kollenda, Sebastian and Hennig, Sven
Journal: ACS chemical biology (2017): 2958--2964
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Spatiotemporal imaging of small GTPases activity in live cells
Authors: Voss, Stephanie and Kr{\"u}ger, Dennis M and Koch, Oliver and Wu, Yao-Wen
Journal: Proceedings of the National Academy of Sciences (2016): 14348--14353
Authors: Voss, Stephanie and Kr{\"u}ger, Dennis M and Koch, Oliver and Wu, Yao-Wen
Journal: Proceedings of the National Academy of Sciences (2016): 14348--14353
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
References
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Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
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The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
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Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
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An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
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Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
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Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
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