Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 651.71 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.179 |
| Extinction coefficient (cm -1 M -1) | 750001 |
| Excitation (nm) | 554 |
| Emission (nm) | 578 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| Tide Fluor™ 3 amine [TF3 amine] *Superior replacement for Cy3* |
| Tide Fluor™ 3 acid [TF3 acid] *Superior replacement for Cy3* |
Related products
| Overview |  SDSProtocol |
See also: Bioconjugation, Dye to Peptide Conjugation, Cell Structures and Organelles, Nucleus, Chemical Reagents, Förster Resonance Energy Transfer (FRET), Peptide and Oligonucleotide Labeling, Digital PCR, DNA and RNA Quantitation, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), Tide Fluor Dyes, PCR Detection of Viral DNA/RNA
Molecular weight 651.71 | Correction Factor (280 nm) 0.179 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 554 | Emission (nm) 578 |
Tide Fluor™ 3 (TF3) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy3. TF3-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy3. In pairing with our Tide Quencher™ 3 (TQ3), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF3 product is used for post-labeling of thiol-modified oligonucleotides and peptides that contain cysteines.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 153.442 µL | 767.212 µL | 1.534 mL | 7.672 mL | 15.344 mL |
| 5 mM | 30.688 µL | 153.442 µL | 306.885 µL | 1.534 mL | 3.069 mL |
| 10 mM | 15.344 µL | 76.721 µL | 153.442 µL | 767.212 µL | 1.534 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.179 |
| Extinction coefficient (cm -1 M -1) | 750001 |
| Excitation (nm) | 554 |
| Emission (nm) | 578 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS* | 341 | 448 | 20000 | 0.187 |
| Tide Fluor™ 2 maleimide [TF2 maleimide] *Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
| Tide Fluor™ 3 azide [TF3 azide] | 554 | 578 | 750001 | 0.179 |
| Tide Fluor™ 3 alkyne [TF3 alkyne] | 554 | 578 | 750001 | 0.179 |
| Tide Fluor™ 5WS maleimide [TF5WS maleimide] *Superior replacement for Cy5* | 649 | 664 | 250000 | 0.027 |
| Tide Fluor™ 4 maleimide [TF4 maleimide] *Superior replacement for ROX and Texas Red* | 578 | 602 | 90000 | 0.436 |
| Tide Fluor™ 6WS maleimide [TF6WS maleimide] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.101 |
| Tide Fluor™ 7WS maleimide [TF7WS maleimide] *Superior replacement for Cy7* | 756 | 780 | 275000 | 0.049 |
| Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission* | 785 | 801 | 250000 | 0.109 |
Show More (4) | ||||
Images
Figure 1. Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Citations
View all 11 citations: Citation Explorer
RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein
Authors: Pantoom, Supansa and Konstantinidis, Georgios and Voss, Stephanie and Han, Hongmei and Hofnagel, Oliver and Li, Zhiyu and Wu, Yao-Wen
Journal: Autophagy (2020): 1--15
Authors: Pantoom, Supansa and Konstantinidis, Georgios and Voss, Stephanie and Han, Hongmei and Hofnagel, Oliver and Li, Zhiyu and Wu, Yao-Wen
Journal: Autophagy (2020): 1--15
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Using a Specific RNA--Protein Interaction To Quench the Fluorescent RNA Spinach
Authors: Roszyk, Laura and Kollenda, Sebastian and Hennig, Sven
Journal: ACS chemical biology (2017): 2958--2964
Authors: Roszyk, Laura and Kollenda, Sebastian and Hennig, Sven
Journal: ACS chemical biology (2017): 2958--2964
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Spatiotemporal imaging of small GTPases activity in live cells
Authors: Voss, Stephanie and Kr{\"u}ger, Dennis M and Koch, Oliver and Wu, Yao-Wen
Journal: Proceedings of the National Academy of Sciences (2016): 14348--14353
Authors: Voss, Stephanie and Kr{\"u}ger, Dennis M and Koch, Oliver and Wu, Yao-Wen
Journal: Proceedings of the National Academy of Sciences (2016): 14348--14353
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
References
View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Rapid detection and quantification of Propionibacteriaceae
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519