Name: Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*
Brand: AAT Bioquest
SKU: 2337
Price: 232 USD
Availability: InStock

Tide Fluor™ 8WS maleimide

TF8WS maleimide; Near Infrared Emission

Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800. Their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family more robust to pH-sensitive assays. In some cases TF8WS-labeled peptides and nucleotides exhibit stronger fluorescence and higher photostability than the ones labeled with IRDye 800. In pairing with our Tide Quencher™ 8WS (TQ8WS), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TQ8WS product is used for post-labeling of thiol-modified oligonucleotides and peptides that contain cysteines.

<p>Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2337	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	69.451 µL	347.256 µL	694.512 µL	3.473 mL	6.945 mL
5 mM	13.89 µL	69.451 µL	138.902 µL	694.512 µL	1.389 mL
10 mM	6.945 µL	34.726 µL	69.451 µL	347.256 µL	694.512 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1439.86 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker

Authors:

Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M

Journal:

Molecular Imaging and Biology (2023): 1--14

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation

Authors:

Perlenfein, Tyler J and Murphy, Regina M

Journal:

Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme

Authors:

Olea, Charles and Joyce, Gerald F

Journal:

Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia

Authors:

Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G

Journal:

American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces

Authors:

Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined

Journal:

ECS Transactions (2015): 9--16

References

View all 25 references: Citation Explorer

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens

Authors:

Yang GP, Erdman DD, Tondella ML, Fields BS.

Journal:

J Virol Methods (2009): 288

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe

Authors:

Andreoni A, Bondani M, Nardo L.

Journal:

Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer

Authors:

Ogawa M, Kosaka N, Choyke PL, Kobayashi H.

Journal:

Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates

Authors:

Kim GI, Kim KW, Oh MK, Sung YM.

Journal:

Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening

Authors:

Yu X, Sainz B, Jr., Uprichard SL.

Journal:

Antimicrob Agents Chemother (2009): 4311

Physical properties

Molecular weight	1439.86
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.109
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	785
Emission (nm)	801
Quantum yield	0.21 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025