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Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*
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Application notes
Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800. Their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family more robust to pH-sensitive assays. In some cases TF8WS-labeled peptides and nucleotides exhibit stronger fluorescence and higher photostability than the ones labeled with IRDye 800. In pairing with our Tide Quencher™ 8WS (TQ8WS), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TQ8WS product is used for post-labeling of thiol-modified oligonucleotides and peptides that contain cysteines.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 69.451 µL | 347.256 µL | 694.512 µL | 3.473 mL | 6.945 mL |
| 5 mM | 13.89 µL | 69.451 µL | 138.902 µL | 694.512 µL | 1.389 mL |
| 10 mM | 6.945 µL | 34.726 µL | 69.451 µL | 347.256 µL | 694.512 µL |
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| Tide Fluor™ 1 maleimide [TF1 maleimide] *Superior replacement for EDANS* | 341 | 448 | 20000 | 0.187 |
| Tide Fluor™ 2 maleimide [TF2 maleimide] *Superior replacement for fluorescein* | 503 | 525 | 75000 | 0.09 |
| Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3* | 554 | 578 | 750001 | 0.179 |
| Tide Fluor™ 5WS maleimide [TF5WS maleimide] *Superior replacement for Cy5* | 649 | 664 | 250000 | 0.027 |
| Tide Fluor™ 4 maleimide [TF4 maleimide] *Superior replacement for ROX and Texas Red* | 578 | 602 | 90000 | 0.436 |
| Tide Fluor™ 6WS maleimide [TF6WS maleimide] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.101 |
| Tide Fluor™ 7WS maleimide [TF7WS maleimide] *Superior replacement for Cy7* | 756 | 780 | 275000 | 0.049 |
| Tide Fluor™ 3WS maleimide [TF3WS maleimide] *Superior replacement for Cy3* | 551 | 563 | 150000 | 0.079 |
| Tide Fluor™ 2WS maleimide [TF2WS Maleimide] *Superior replacement for FITC* | 491 | 516 | 75000 | 0.11 |
Citations
View all 8 citations: Citation Explorer
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular Imaging and Biology (2023): 1--14
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular Imaging and Biology (2023): 1--14
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
References
View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311