TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*
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Additional ordering information
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Physical properties
Molecular weight | 500.93 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 552 |
Emission (nm) | 574 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
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See also: Mitochondria, Rhodamines and Rhodamine Derivatives, Flow Cytometry Reagents, Mitochondrial Membrane Potential
CAS 115532-50-8 | Molecular weight 500.93 | Excitation (nm) 552 | Emission (nm) 574 |
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 199.629 µL | 998.143 µL | 1.996 mL | 9.981 mL | 19.963 mL |
5 mM | 39.926 µL | 199.629 µL | 399.257 µL | 1.996 mL | 3.993 mL |
10 mM | 19.963 µL | 99.814 µL | 199.629 µL | 998.143 µL | 1.996 mL |
Molarity calculator
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Citations
View all 13 citations: Citation Explorer
Chronic calcium signaling in IgE+ B cells limits plasma cell differentiation and survival
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Immunity (2021): 2756--2771
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Immunity (2021): 2756--2771
Genome-wide screens identify calcium signaling as a key regulator of IgE+ plasma cell differentiation and survival
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Available at SSRN 3800380 (2021)
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Available at SSRN 3800380 (2021)
A Clickable APEX Probe for Proximity-Dependent Proteomic Profiling in Yeast
Authors: Li, Yi and Tian, Caiping and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: Cell Chemical Biology (2020)
Authors: Li, Yi and Tian, Caiping and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: Cell Chemical Biology (2020)
Protocol for Proximity-Dependent Proteomic Profiling in Yeast Cells by APEX and Alk-Ph Probe
Authors: Li, Yi and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: STAR protocols (2020): 100137
Authors: Li, Yi and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: STAR protocols (2020): 100137
Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis
Authors: Tan, Shougang and Hou, Xiaoqun and Mei, Lin
Journal: Oncology Letters (2020): 1--1
Authors: Tan, Shougang and Hou, Xiaoqun and Mei, Lin
Journal: Oncology Letters (2020): 1--1
Low-dose sorafenib acts as a mitochondrial uncoupler and ameliorates nonalcoholic steatohepatitis
Authors: Jian, Chongshu and Fu, Jiajun and Cheng, Xu and Shen, Li-Jun and Ji, Yan-Xiao and Wang, Xiaoming and Pan, Shan and Tian, Han and Tian, Song and Liao, Rufang and others,
Journal: Cell metabolism (2020): 892--908
Authors: Jian, Chongshu and Fu, Jiajun and Cheng, Xu and Shen, Li-Jun and Ji, Yan-Xiao and Wang, Xiaoming and Pan, Shan and Tian, Han and Tian, Song and Liao, Rufang and others,
Journal: Cell metabolism (2020): 892--908
Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production
Authors: Wang, Ruifang and Zhu, Yinxing and Lin, Xian and Ren, Chenwei and Zhao, Jiachang and Wang, Fangfang and Gao, Xiaochen and Xiao, Rong and Zhao, Lianzhong and Chen, Huanchun and others,
Journal: Autophagy (2019): 1163--1181
Authors: Wang, Ruifang and Zhu, Yinxing and Lin, Xian and Ren, Chenwei and Zhao, Jiachang and Wang, Fangfang and Gao, Xiaochen and Xiao, Rong and Zhao, Lianzhong and Chen, Huanchun and others,
Journal: Autophagy (2019): 1163--1181
Thiolated nanoparticles overcome the mucus barrier and epithelial barrier for oral delivery of insulin
Authors: Zhou, Shurong and Deng, Hailiang and Zhang, Yang and Wu, Peiyao and He, Bing and Dai, Wenbing and Zhang, Hua and Zhang, Qiang and Zhao, Rongsheng and Wang, Xueqing
Journal: Molecular Pharmaceutics (2019)
Authors: Zhou, Shurong and Deng, Hailiang and Zhang, Yang and Wu, Peiyao and He, Bing and Dai, Wenbing and Zhang, Hua and Zhang, Qiang and Zhao, Rongsheng and Wang, Xueqing
Journal: Molecular Pharmaceutics (2019)
Augmenter of liver regeneration promotes mitochondrial biogenesis in renal ischemia--reperfusion injury
Authors: Huang, Li-li and Long, Rui-ting and Jiang, Gui-ping and Jiang, Xiao and Sun, Hang and Guo, Hui and Liao, Xiao-hui
Journal: Apoptosis (2018): 1--12
Authors: Huang, Li-li and Long, Rui-ting and Jiang, Gui-ping and Jiang, Xiao and Sun, Hang and Guo, Hui and Liao, Xiao-hui
Journal: Apoptosis (2018): 1--12
Aryl-and alkyl-phosphorus-containing flame retardants induced mitochondrial impairment and cell death in Chinese hamster ovary (CHO-k1) cells
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
References
View all 73 references: Citation Explorer
CRYAB and HSPB2 deficiency increases myocyte mitochondrial permeability transition and mitochondrial calcium uptake
Authors: Kadono T, Zhang XQ, Srinivasan S, Ishida H, Barry WH, Benjamin IJ.
Journal: J Mol Cell Cardiol (2006): 783
Authors: Kadono T, Zhang XQ, Srinivasan S, Ishida H, Barry WH, Benjamin IJ.
Journal: J Mol Cell Cardiol (2006): 783
Dystrophin is a possible end-target of ischemic preconditioning against cardiomyocyte oncosis during the early phase of reperfusion
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Adaphostin and other anticancer drugs quench the fluorescence of mitochondrial potential probes
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Evaluation of the reactivity of apoptosis markers before and after cryopreservation in cord blood CD34(+) cells
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Overexpression of inducible heat shock protein 70 and its mutants in astrocytes is associated with maintenance of mitochondrial physiology during glucose deprivation stress
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NECA at reperfusion limits infarction and inhibits formation of the mitochondrial permeability transition pore by activating p70S6 kinase
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Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation
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High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening
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Journal: Arch Toxicol (2006): 580
Mitochondrial Ca2+ uptake during simulated ischemia does not affect permeability transition pore opening upon simulated reperfusion
Authors: Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.
Journal: Cardiovasc Res (2006): 715
Authors: Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.
Journal: Cardiovasc Res (2006): 715
Application notes
FAQ
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Why is determining cell viability important?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives for ethidium bromide in agarose gels?
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Why is determining cell viability important?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?