TMRM [Tetramethylrhodamine methyl ester] CAS#: 115532-50-8

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Physical properties

Molecular weight	500.93
Solvent	DMSO

Spectral properties

Excitation (nm)	552
Emission (nm)	574

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

115532-50-8

Molecular weight

500.93

Excitation (nm)

552

Emission (nm)

574

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	199.629 µL	998.143 µL	1.996 mL	9.981 mL	19.963 mL
5 mM	39.926 µL	199.629 µL	399.257 µL	1.996 mL	3.993 mL
10 mM	19.963 µL	99.814 µL	199.629 µL	998.143 µL	1.996 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	552
Emission (nm)	574

Images

Figure 1. Chemical structure for TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*

Citations

View all 13 citations: Citation Explorer

Chronic calcium signaling in IgE+ B cells limits plasma cell differentiation and survival
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Immunity (2021): 2756--2771

Genome-wide screens identify calcium signaling as a key regulator of IgE+ plasma cell differentiation and survival
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Available at SSRN 3800380 (2021)

A Clickable APEX Probe for Proximity-Dependent Proteomic Profiling in Yeast
Authors: Li, Yi and Tian, Caiping and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: Cell Chemical Biology (2020)

Protocol for Proximity-Dependent Proteomic Profiling in Yeast Cells by APEX and Alk-Ph Probe
Authors: Li, Yi and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: STAR protocols (2020): 100137

Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis
Authors: Tan, Shougang and Hou, Xiaoqun and Mei, Lin
Journal: Oncology Letters (2020): 1--1

Low-dose sorafenib acts as a mitochondrial uncoupler and ameliorates nonalcoholic steatohepatitis
Authors: Jian, Chongshu and Fu, Jiajun and Cheng, Xu and Shen, Li-Jun and Ji, Yan-Xiao and Wang, Xiaoming and Pan, Shan and Tian, Han and Tian, Song and Liao, Rufang and others,
Journal: Cell metabolism (2020): 892--908

Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production
Authors: Wang, Ruifang and Zhu, Yinxing and Lin, Xian and Ren, Chenwei and Zhao, Jiachang and Wang, Fangfang and Gao, Xiaochen and Xiao, Rong and Zhao, Lianzhong and Chen, Huanchun and others,
Journal: Autophagy (2019): 1163--1181

Thiolated nanoparticles overcome the mucus barrier and epithelial barrier for oral delivery of insulin
Authors: Zhou, Shurong and Deng, Hailiang and Zhang, Yang and Wu, Peiyao and He, Bing and Dai, Wenbing and Zhang, Hua and Zhang, Qiang and Zhao, Rongsheng and Wang, Xueqing
Journal: Molecular Pharmaceutics (2019)

Augmenter of liver regeneration promotes mitochondrial biogenesis in renal ischemia--reperfusion injury
Authors: Huang, Li-li and Long, Rui-ting and Jiang, Gui-ping and Jiang, Xiao and Sun, Hang and Guo, Hui and Liao, Xiao-hui
Journal: Apoptosis (2018): 1--12

Aryl-and alkyl-phosphorus-containing flame retardants induced mitochondrial impairment and cell death in Chinese hamster ovary (CHO-k1) cells
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786

References

View all 73 references: Citation Explorer

CRYAB and HSPB2 deficiency increases myocyte mitochondrial permeability transition and mitochondrial calcium uptake
Authors: Kadono T, Zhang XQ, Srinivasan S, Ishida H, Barry WH, Benjamin IJ.
Journal: J Mol Cell Cardiol (2006): 783

Dystrophin is a possible end-target of ischemic preconditioning against cardiomyocyte oncosis during the early phase of reperfusion
Authors: Kyoi S, Otani H, Hamano A, Matsuhisa S, Akita Y, Fujiwara H, Hattori R, Imamura H, Kamihata H, Iwasaka T.
Journal: Cardiovasc Res (2006): 354

Adaphostin and other anticancer drugs quench the fluorescence of mitochondrial potential probes
Authors: Le SB, Holmuhamedov EL, Narayanan VL, Sausville EA, Kaufmann SH.
Journal: Cell Death Differ (2006): 151

Evaluation of the reactivity of apoptosis markers before and after cryopreservation in cord blood CD34(+) cells
Authors: Greco NJ, Seetharaman S, Kurtz J, Lee WR, Moroff G.
Journal: Stem Cells Dev (2006): 124

Overexpression of inducible heat shock protein 70 and its mutants in astrocytes is associated with maintenance of mitochondrial physiology during glucose deprivation stress
Authors: Ouyang YB, Xu LJ, Sun YJ, Giffard RG.
Journal: Cell Stress Chaperones (2006): 180

Fatty acid ethyl esters cause pancreatic calcium toxicity via inositol trisphosphate receptors and loss of ATP synthesis
Authors: Criddle DN, Murphy J, Fistetto G, Barrow S, Tepikin AV, Neoptolemos JP, Sutton R, Petersen OH.
Journal: Gastroenterology (2006): 781

NECA at reperfusion limits infarction and inhibits formation of the mitochondrial permeability transition pore by activating p70S6 kinase
Authors: Forster K, Paul I, Solenkova N, Staudt A, Cohen MV, Downey JM, Felix SB, Krieg T.
Journal: Basic Res Cardiol (2006): 319

Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation
Authors: Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.
Journal: Eur J Pharmacol (2006): 69

High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening
Authors: O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.
Journal: Arch Toxicol (2006): 580

Mitochondrial Ca2+ uptake during simulated ischemia does not affect permeability transition pore opening upon simulated reperfusion
Authors: Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.
Journal: Cardiovasc Res (2006): 715