trFluor™ Tb succinimidyl ester

Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences. Our trFluor™ Tb probes enable time-resolved fluorometry (TRF) for the assays that require high sensitivity. These trFluor™ Tb probes have large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our trFluor™ Tb probes have relatively high stability, high emission yield and ability to be linked to biomolecules. Moreover, our trFluor™ Tb probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies.

<p>Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1443	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of trFluor™ Tb succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	64.404 µL	322.022 µL	644.044 µL	3.22 mL	6.44 mL
5 mM	12.881 µL	64.404 µL	128.809 µL	644.044 µL	1.288 mL
10 mM	6.44 µL	32.202 µL	64.404 µL	322.022 µL	644.044 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1552.69 g/mol	=	mL	x	mM	=	mmol

References

View all 61 references: Citation Explorer

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors

Authors:

Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.

Journal:

Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays

Authors:

Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.

Journal:

Assay Drug Dev Technol. (2012)

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach

Authors:

Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.

Journal:

J Phys Chem B (2011): 11439

A homogeneous single-label time-resolved fluorescence cAMP assay

Authors:

Martikkala E, Rozw and owicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.

Journal:

J Biomol Screen (2011): 356

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies

Authors:

Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.

Journal:

J Biol Chem (2011): 11337

Physical properties

Molecular weight	1552.69
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.942
Correction factor (280 nm)	0.797
Excitation (nm)	333
Emission (nm)	544

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 23, 2025