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TRITC-DHPE
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Application notes
TRITC DHPE is a phospholipid that is labeled on the head group with the bright, red-fluorescent TRITC dye. Rhodamine-labeled phospholipids have been used as tracers for membrane traffic during endocytosis and for lipid processing in hepatocytes. TRITC DHPE can be used as an energy transfer acceptor from NBD, BODIPY and fluorescein lipid probes. The longer emission wavelength of the TRITC dye provides superior separation of the donor and acceptor emission signals in resonance energy transfer microscopy.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of TRITC-DHPE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 80.861 µL | 404.305 µL | 808.61 µL | 4.043 mL | 8.086 mL |
| 5 mM | 16.172 µL | 80.861 µL | 161.722 µL | 808.61 µL | 1.617 mL |
| 10 mM | 8.086 µL | 40.431 µL | 80.861 µL | 404.305 µL | 808.61 µL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2* | 544 | 570 | 100000 | 0.27 | 0.34 |
| 6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5* | 544 | 570 | 100000 | 0.27 | 0.34 |
| Fluorescein DHPE | 498 | 517 | 800001 | 0.32 | 0.35 |
| OG488 DHPE | 498 | 526 | 76000 | 0.31 | 0.12 |
Citations
View all 4 citations: Citation Explorer
Highly Robust Multilamellar Lipid Vesicles Generated through Intervesicular Self-assembly Mediated by Hydrolyzed Collagen Peptides
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)
The magnitude of LFA-1/ICAM-1 forces fine-tune TCR-triggered T cell activation
Authors: Ma, Victor Pui-Yan and Hu, Yuesong and Kellner, Anna V and Brockman, Joshua M and Velusamy, Arventh and Blanchard, Aaron T and Evavold, Brian D and Alon, Ronen and Salaita, Khalid
Journal: Science advances (2022): eabg4485
Authors: Ma, Victor Pui-Yan and Hu, Yuesong and Kellner, Anna V and Brockman, Joshua M and Velusamy, Arventh and Blanchard, Aaron T and Evavold, Brian D and Alon, Ronen and Salaita, Khalid
Journal: Science advances (2022): eabg4485
Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)
Biomimetic Multilayered Lipid Nanovesicles for Potent Protein Vaccination
Authors: Koo, Bon Il and Lee, Dong Jae and Rahman, Rafia Tasnim and Nam, Yoon Sung
Journal: Advanced Healthcare Materials : 2304109
Authors: Koo, Bon Il and Lee, Dong Jae and Rahman, Rafia Tasnim and Nam, Yoon Sung
Journal: Advanced Healthcare Materials : 2304109
References
View all 27 references: Citation Explorer
Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221