Name: XFD350 Anti-human CD45 Antibody *HI30*
Brand: AAT Bioquest
SKU: 10450140
Availability: InStock

XFD350 Anti-human CD45 Antibody

HI30

The HI30 monoclonal antibody recognizes human CD45, a 180 - 240 kD transmembrane glycoprotein typically found on the surface of B cells, neutrophils, hematopoietic cells and dendritic cells. CD45 is a component of vital cellular pathways, in particular, the regulation of receptor signaling pathway via JAK-STAT, B cell receptor signaling pathway and T cell receptor signaling pathway. Also, in certain organisms, it plays a role in the downregulation of cytokine-mediated signaling pathway, represses interleukin-2 biosynthetic process and is an enhancer of humoral immune response mediated by circulating immunoglobulin. CD45 has been thought to be involved with critical biological processes such as dephosphorylation, especially protein dephosphorylation, and is associated with a variety of biologically interesting macromolecules/ligands, for instance, p56lck. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of neuroscience, immunology and neuroscience cell markers. This antibody was purified through affinity chromatography and conjugated to XFD350 (ex/em = 343/441 nm). XFD350 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 350 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 355 nm laser and 450/50 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).

Protocol

COA

Catalog	Size	Price	Quantity
10450140	100 tests	Price
10450141	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd45 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leukocyte Common Antigen (LCA), T200, PTPRC
Clone	HI30
Host	Mouse
Isotype	Mouse IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.19
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	343
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet (Catalog 10450140)
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on December 18, 2025