Name: XFD350 Anti-human CD53 Antibody *HI29*
Brand: AAT Bioquest
SKU: 10530140
Availability: InStock

XFD350 Anti-human CD53 Antibody

HI29

HI29 is an anti-human monoclonal antibody that targets the CD53 antigen. CD53 (sometimes referred to as Tetraspanin-25, MOX44 or OX44) is a 35 - 42 kD member of the tetraspan family that is found on the surface of cells such as NK cells. In some organisms, CD53 enhances myoblast fusion, and is associated with a variety of biologically interesting macromolecules/ligands, in particular, VLA-4, Integrins and HLA-DR. CD53 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD53 has a variety of applications in costimulatory molecules research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD350 (ex/em = 343/441 nm). XFD350 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 350 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 355 nm laser and 450/50 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).

Datasheet

COA

Catalog	Size	Price	Quantity
10530140	100 tests	Price
10530141	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD53 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Tetraspanin-25, MOX44
Clone	HI29
Host	Mouse
Isotype	IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.19
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	343
Emission (nm)	441

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on August 11, 2026