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XFD350 Anti-human CD55 Antibody
HI55a
HI55a is an anti-human monoclonal antibody that targets the CD55 antigen. CD55 (sometimes called Complement decay accelerating factor or DAF (Decay Accelerating Factor)) is a 60 - 70 kD single-pass type i membrane protein that is found on the surface of cells like macrophages, platelets, granulocytes, T cells and NK cells. CD55 is a component of vital cellular pathways, for example, the complement activation, classical pathway and regulation of lipopolysaccharide-mediated signaling pathway. Additionally, in some organisms, it is involved in the positive regulation of CD4-positive, alpha-beta T cell activation, upregulates CD4-positive, alpha-beta T cell proliferation and acts to positively regulate cytosolic calcium ion concentration. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like SCR, CD97 and Echoviruses. CD55 is a fairly uncommon antibody target, with a little more than 3000 publications in the last decade. Even still, CD55 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of cell biology, neuroinflammation and immunology. This antibody was purified through affinity chromatography and conjugated to XFD350 (ex/em = 343/441 nm). XFD350 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 350 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 355 nm laser and 447/60 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10550140 | 100 tests | Price | |
| 10550141 | 500 tests | Price |
Antibody properties
| Other names | DAF |
| Clone | HI55a |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.25 |
| Correction factor (280 nm) | 0.19 |
| Extinction coefficient (cm -1 M -1) | 19000 |
| Excitation (nm) | 343 |
| Emission (nm) | 441 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on December 21, 2025