Name: XFD350 Anti-human/monkey CD154 Antibody *5C8*
Brand: AAT Bioquest
SKU: 11540140
Availability: InStock

XFD350 Anti-human/monkey CD154 Antibody

5C8

5C8 is an anti-human/monkey monoclonal antibody that forms an immune complex with the CD154 antigen. CD154 (also known as gp39) is a 32 - 39 kD member of the TNF superfamily that is expressed on the surface of cells such as T cells. CD154 is associated with a variety of biologically interesting macromolecules/ligands, for instance, CD40. CD154 is a fairly uncommon antibody target, with a little more than 4000 publications in the last decade. Even still, CD154 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to XFD350 (ex/em = 343/441 nm). XFD350 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 350 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 355 nm laser and 450/50 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Datasheet

COA

Catalog	Size	Price	Quantity
11540140	100 tests	Price
11540141	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD154 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	CD40L, gp39, TRAP, T-BAM
Clone	5C8
Host	Mouse
Isotype	IgG2a
Reactivity	Human, Monkey
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.19
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	343
Emission (nm)	441

Usage and storage

Intended use

Research Use Only (RUO)

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Email	sales@aatbio.com
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Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 9, 2026