Name: XFD488 Anti-human CD4 Antibody *OKT-4*
Brand: AAT Bioquest
SKU: 10043150
Availability: InStock

XFD488 Anti-human CD4 Antibody

OKT-4

OKT-4 is an anti-human monoclonal antibody that targets the CD4 antigen. CD4 (also known as T4) is a 55 kD member of the Ig superfamily that is located on the surface of cells such as macrophages. In certain organisms, CD4 positively regulates kinase activity, promotes I-kappaB kinase/NF-kappaB signaling and upregulates transcription, DNA-templated. Additionally, it has been thought to be involved with essential biological processes such as immune response, especially adaptive immune response. CD4 is a member of critical cellular pathways, for instance, the cytokine-mediated signaling pathway, enzyme linked receptor protein signaling pathway and interleukin-15-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like Lck and IL-16. CD4 is a very popular antibody target, with over 185000 publications in the last decade. CD4 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD488 (ex/em = 499/520 nm). XFD488 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 488 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 488 nm laser and 525/50 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded tonsil cancer tissue using PSA™ amplification method. Tonsil cancer tissue sections were stained with XFD488 Anti-human CD4 Antibody (Cat #10043150) and then incubated with HRP tagged anti-XFD488 secondary antibody followed by iFluor® 647 Styramide™ (Cat# 45027). The sections were counterstained with Hoechst 33342 (Cat# 17535) and imaged under DAPI and Cy5 filters.

Datasheet

COA

Catalog	Size	Price	Quantity
10043150	100 tests	Price
10043151	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD4 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leu-3, T4
Clone	OKT-4
Host	Mouse
Isotype	IgG2b, kappa
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

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Email	sales@aatbio.com
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Custom size	Inquire
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Shipping	Standard overnight for United States, inquire for international

Page updated on April 13, 2026