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XFD488 Anti-human CD47 Antibody
HI172
HI172 is an anti-human monoclonal antibody that is specific for the CD47 antigen. CD47 (sometimes called Integrin associated protein) is a 42 - 52 kD multi-pass membrane protein that is found on the surface of cells such as granulocytes, platelets, endothelial cells and B cells. CD47 acts in critical cellular pathways, in particular, the negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis and integrin-mediated signaling pathway. In addition, in certain organisms, it downregulates Fc-gamma receptor signaling pathway involved in phagocytosis, upregulates T cell activation and acts to positively regulate inflammatory response. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like SIRP, Thrombospondin and CD61. CD47 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD47 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD488 (ex/em = 499/520 nm). XFD488 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 488 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 488 nm laser and 525/40 nm bandpass filter (for example, as in the Beckman Coulter DxFLEX).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10471150 | 100 tests | Price | |
| 10471151 | 500 tests | Price |
Antibody properties
| Other names | gp42, IAP, neurophilin, MER6, Integrin associated protein |
| Clone | HI172 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.30 |
| Correction factor (280 nm) | 0.11 |
| Extinction coefficient (cm -1 M -1) | 71000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.92 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on December 21, 2025