Name: XFD488 Anti-human CD58 Antibody *HI58a*
Brand: AAT Bioquest
SKU: 10580150
Availability: InStock

XFD488 Anti-human CD58 Antibody

HI58a

HI58a is an anti-human monoclonal antibody that targets the CD58 antigen. CD58 (sometimes referred to as Lymphocyte function-associated antigen 3, LFA3 or LFA-3) is a 45 - 70 kD transmembrane protein that is located on the surface of cells such as macrophages, NK cells, dendritic cells, B cells and granulocytes. CD58 has been associated with key biological processes such as cell-cell adhesion, especially heterotypic cell-cell adhesion. Also, in certain organisms, it is involved in the positive regulation of interleukin-8 secretion. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like CD2 and LFA-2. CD58 is a relatively rare antibody target, with less than 1000 publications in the last decade. Even still, CD58 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of cell biology. This antibody was purified through affinity chromatography and conjugated to XFD488 (ex/em = 499/520 nm). XFD488 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 488 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).

Datasheet

COA

Catalog	Size	Price	Quantity
10580150	100 tests	Price
10580151	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD58 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	LFA-3
Clone	HI58a
Host	Mouse
Isotype	IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on August 12, 2026