Name: XFD555 Anti-human CD279 Antibody *J116*
Brand: AAT Bioquest
SKU: 12793160
Availability: InStock

XFD555 Anti-human CD279 Antibody

J116

The J116 monoclonal antibody binds to human CD279, a 50 - 55 kD member of the Ig superfamily commonly located on the surface of B cells and thymocytes. CD279 is associated with a variety of biologically interesting macromolecules/ligands, for instance, PDL1. CD279 is a relatively rare antibody target, with fewer than 1000 publications in the last decade. Even still, CD279 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of inhibitory molecules, cancer biomarkers and immunology. This antibody was purified through affinity chromatography and conjugated to XFD555 (ex/em = 553/568 nm). XFD555 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 555 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 561 nm laser and 586/20 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte).

Datasheet

COA

Catalog	Size	Price	Quantity
12793160	100 tests	Price
12793161	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD279 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Physical properties

Molecular weight	N/A
Solvent	water

Antibody properties

Other names	PD1
Clone	J116
Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.08
Correction factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	553
Emission (nm)	568
Quantum yield	0.1 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 11, 2026