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XFD555 Anti-human CD44 Antibody
HI44a
The HI44a monoclonal antibody reacts with human CD44, a 85 kD transmembrane glycoprotein commonly expressed on the surface of leukocytes, endothelial cells, lymphohematopoietic cells, epithelial cells and hepatocytes. In many organisms, CD44 acts to positively regulate peptidyl-tyrosine phosphorylation, enhances peptidyl-serine phosphorylation and is a positive regulator of ERK1 and ERK2 cascade. Also, it acts in critical cellular pathways, namely, the interferon-gamma-mediated signaling pathway and negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as Matrix metalloprotein- ases (MMPs), Collagen, Osteopontin and Hyaluronan. CD44 is a very popular antibody target, with over 40000 publications in the last decade. CD44 has been widely used in immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD555 (ex/em = 553/568 nm). XFD555 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 555 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 561 nm laser and 585/20 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10440160 | 100 tests | Price | |
| 10440161 | 500 tests | Price |
Antibody properties
| Other names | ECMR-III, Pgp-1, HUTCH-1, H-CAM |
| Clone | HI44a |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.08 |
| Correction factor (280 nm) | 0.08 |
| Extinction coefficient (cm -1 M -1) | 150000 |
| Excitation (nm) | 553 |
| Emission (nm) | 568 |
| Quantum yield | 0.1 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on December 21, 2025