Name: XFD555 Anti-human CD99 Antibody *HIT4*
Brand: AAT Bioquest
SKU: 10991160
Availability: InStock

XFD555 Anti-human CD99 Antibody

The HIT4 monoclonal antibody reacts with human CD99, a transmembrane glycoprotein commonly located on the surface of epithelial cells, t cells, lymphocytes, natural killer cells and monocytes. In some organisms, CD99 positively regulates neutrophil extravasation, and is associated with a variety of biologically interesting macromolecules/ligands, for instance, . CD99 is a fairly uncommon antibody target, with a little more than 3000 publications in the last decade. Even still, CD99 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD555 (ex/em = 553/568 nm). XFD555 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 555 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 561 nm laser and 583/24 nm bandpass filter (for example, as in the Luminex Amnis CellStream).

Datasheet

COA

Catalog	Size	Price	Quantity
10991160	100 tests	Price
10991161	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd99 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	MIC2, E2 antigen, HBA71, MSK5X
Clone	HIT4
Host	Mouse
Isotype	Mouse IgM
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.08
Correction factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	553
Emission (nm)	568
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on March 22, 2026