XFD594 Anti-human CD116 Antibody *4H1, XFD594 Same Structure to Alexa Fluor™ 594*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Absorbance (nm) | 592 |
Correction Factor (260 nm) | 0.43 |
Correction Factor (280 nm) | 0.56 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 590 |
Emission (nm) | 618 |
Quantum yield | 0.661 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names GM-CSFRα chain | Clone 4H1 | Host mouse | Isotype Mouse IgG1 kappa | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Absorbance (nm) 592 | Correction Factor (260 nm) 0.43 | Correction Factor (280 nm) 0.56 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 590 | Emission (nm) 618 | Quantum yield 0.661 |
The 4H1 monoclonal antibody binds to human CD116, a 70 - 85 kD glycoprotein commonly located on the surface of endothelial precursors and macrophages. CD116 is associated with a variety of biologically interesting macromolecules/ligands, for instance, CD131 and GM-CSF. CD116 is a relatively rare antibody target, with fewer than 100 publications in the last decade. Even still, CD116 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD594 (ex/em = 590/618 nm). XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 592 nm laser and 610/30 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 592 |
Correction Factor (260 nm) | 0.43 |
Correction Factor (280 nm) | 0.56 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 590 |
Emission (nm) | 618 |
Quantum yield | 0.661 |
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd116 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?