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XFD594 Anti-human CD16 Antibody
3G8
The 3G8 monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils. CD16 is a member of key cellular pathways, namely, the Fc-gamma receptor signaling pathway involved in phagocytosis. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like IgG Fc. CD16 is a very popular antibody target, with over 25000 publications in the last decade. CD16a is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD594 (ex/em = 590/618 nm). XFD594 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 594 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 592 nm laser and 610/30 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10161170 | 100 tests | Price | |
| 10161171 | 500 tests | Price |
Physical properties
| Molecular weight | N/A |
| Solvent | water |
Antibody properties
| Other names | FCRIIIA |
| Clone | 3G8 |
| Host | Mouse |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 592 |
| Correction factor (260 nm) | 0.43 |
| Correction factor (280 nm) | 0.56 |
| Extinction coefficient (cm -1 M -1) | 90000 |
| Excitation (nm) | 590 |
| Emission (nm) | 618 |
| Quantum yield | 0.66 1 |
Usage and storage
| Intended use | Research Use Only (RUO) |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on August 10, 2026