Name: XFD594 Anti-human CD19 Antibody *4G7*
Brand: AAT Bioquest
SKU: 10193170
Availability: InStock

XFD594 Anti-human CD19 Antibody

4G7

The 4G7 monoclonal antibody binds with human CD19, a 95 kD transmembrane protein commonly expressed on the surface of B cells and lymphocytes. CD19 plays a role in essential cellular pathways, for example, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. Moreover, in some organisms, it acts to positively regulate phosphatidylinositol 3-kinase activity, is an enhancer of protein kinase B signaling and is involved in the positive regulation of release of sequestered calcium ion into cytosol. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as Fyn and PI3-kinase. CD19 is a very popular antibody target, with over 30000 publications in the last decade. CD19 is essential for immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD594 (ex/em = 590/618 nm). XFD594 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 594 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 592 nm laser and 610/30 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Datasheet

COA

Catalog	Size	Price	Quantity
10193170	100 tests	Price
10193171	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD19 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	B4
Clone	4G7
Host	Mouse
Isotype	IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	592
Correction factor (260 nm)	0.43
Correction factor (280 nm)	0.56
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	590
Emission (nm)	618
Quantum yield	0.66 ¹

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on August 20, 2026