Name: XFD594 Anti-human CD28 Antibody *CD28.2*
Brand: AAT Bioquest
SKU: 10280170
Availability: InStock

XFD594 Anti-human CD28 Antibody

CD28.2

CD28.2 is an anti-human monoclonal antibody that forms an immune complex with the CD28 antigen. CD28 (sometimes called T44) is a 44 kD member of the Ig superfamily that is located on the surface of cells such as T cells. CD28 acts in essential cellular pathways, in particular, the cell surface receptor signaling pathway, T cell receptor signaling pathway and apoptotic signaling pathway. Furthermore, in some organisms, it positively regulates isotype switching to IgG isotypes, is an enhancer of interleukin-4 production and upregulates translation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as PI3-kinase, CD80 and CD86. CD28 is a very popular antibody target, with over 30000 publications in the last decade. CD28 is essential for costimulatory molecules and immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD594 (ex/em = 590/618 nm). XFD594 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 594 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 592 nm laser and 610/30 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Datasheet

COA

Catalog	Size	Price	Quantity
10280170	100 tests	Price
10280171	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD28 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Tp44, T44
Clone	CD28.2
Host	Mouse
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	592
Correction factor (260 nm)	0.43
Correction factor (280 nm)	0.56
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	590
Emission (nm)	618
Quantum yield	0.66 ¹

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on July 31, 2026