Name: XFD594 Anti-human/ non-human primates CD137 Antibody *4B4-1*
Brand: AAT Bioquest
SKU: 11370160
Availability: InStock

XFD594 Anti-human/ non-human primates CD137 Antibody

4B4-1

The 4B4-1 monoclonal antibody reacts with human/ non-human primates CD137, a 30 kD member of the TNFR superfamily frequently located on the surface of T cells and follicular dendritic cells. CD137 acts in vital cellular pathways, for example, the tumor necrosis factor-mediated signaling pathway. In addition, in certain organisms, it downregulates cell population proliferation. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as 4-1BB ligand. CD137 is a fairly uncommon antibody target, with a little more than 4100 publications in the last decade. Even still, CD137 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to XFD594 (ex/em = 590/618 nm). XFD594 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 594 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 592 nm laser and 610/30 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Datasheet

COA

Catalog	Size	Price	Quantity
11370160	100 tests	Price
11370161	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD137 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	4-1BB, ILA, TNFRSF9
Clone	4B4-1
Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human, Non-Human Primates
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	592
Correction factor (260 nm)	0.43
Correction factor (280 nm)	0.56
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	590
Emission (nm)	618
Quantum yield	0.66 ¹

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on August 19, 2026