Name: XFD647 Anti-mouse CD49 Antibody *5H10-27 (MFR5)*
Brand: AAT Bioquest
SKU: 10493180
Availability: InStock

XFD647 Anti-mouse CD49 Antibody

5H10-27 (MFR5)

5H10-27 (MFR5) is an anti-mouse monoclonal antibody that forms an immune complex with the CD49e antigen. CD49e (also known as ITGA5, Integrin α5 chain or VLA-5α) is a 135 kD member of the Integrin family that is found on the surface of cells like stem cells and T cells. CD49 is associated with a variety of biologically interesting macromolecules/ligands, for example, fibronectin. CD49 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD49e is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of cell biology. This antibody was purified through affinity chromatography and conjugated to XFD647 (ex/em = 650/671 nm). XFD647 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 647 (Alexa Fluor® is the trademark of Thermo Fisher). It is compatible with the 642 nm laser and 702/85 nm bandpass filter (for example, as in the Luminex Amnis FlowSight).

Protocol

COA

Catalog	Size	Price	Quantity
10493180	100 tests	Price
10493181	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd49e antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	VLA-5α, Integrin α5 chain, ITGA5
Clone	5H10-27 (MFR5)
Host	Rat
Isotype	Rat IgG2a kappa
Reactivity	Mouse
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.00
Correction factor (280 nm)	0.03
Extinction coefficient (cm ^-1 M ^-1)	239000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet (Catalog 10493180)
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on December 20, 2025