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AAT Bioquest

XFD680 Anti-human CD19 Antibody *4G7, XFD680 Same Structure to Alexa Fluor™ 680*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)184000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
B4
Clone
4G7
Host
mouse
Isotype
mouse igg1
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.00
Correction Factor (280 nm)
0.05
Extinction coefficient (cm -1 M -1)
184000
Excitation (nm)
681
Emission (nm)
704
Quantum yield
0.361
The 4G7 monoclonal antibody binds with human CD19, a 95 kD transmembrane protein commonly expressed on the surface of B cells and lymphocytes. CD19 plays a role in essential cellular pathways, for example, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. Moreover, in some organisms, it acts to positively regulate phosphatidylinositol 3-kinase activity, is an enhancer of protein kinase B signaling and is involved in the positive regulation of release of sequestered calcium ion into cytosol. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as Fyn and PI3-kinase. CD19 is a very popular antibody target, with over 30000 publications in the last decade. CD19 is essential for immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to XFD680 (ex/em = 681/704 nm). XFD680 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 680 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 642 nm laser and 664/20 nm bandpass filter (for example, as in the Luminex Guava easyCyte).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.00
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)184000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd19 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.